| ## EMBOSS: geecee

# Program geecee

## Function

Calculates the fractional GC content of nucleic acid sequences

## Description

This calculates the fraction of G+C bases of the input nucleic acid sequence(s).

It reads in nucleic acid sequences, sums the number of 'G' and 'C' bases and writes out the result as the fraction (in the interval 0.0 to 1.0) of the length of the whole sequence.

## Usage

Here is a sample session with geecee.

    % geecee embl:hhtetra
    Output file [hhtetra.geecee]:

## Command line arguments

       Mandatory qualifiers:
      [-sequence]          seqall     Sequence database USA
      [-outfile]           outfile    Output file name

       Optional qualifiers: (none)
       Advanced qualifiers: (none)
       General qualifiers:
      -help                bool       report command line options. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose

Mandatory qualifiers | Allowed values | Default
--- | --- | ---
[-sequence] (Parameter 1) | Sequence database USA | Readable sequence(s) | Required
[-outfile] (Parameter 2) | Output file name | Output file | <sequence>.geecee
Optional qualifiers | Allowed values | Default
(none)
Advanced qualifiers | Allowed values | Default
(none)

## Input file format

Any DNA sequence USA.

## Output file format

Here is the output from the example usage:

    #Sequence   GC content
    HHTETRA       0.53

The first non-blank line is the title line. Subsequent lines consist of two columns of data.

- The first column is the name of the sequence.
- The second column is the percentage G+C content of the sequence.

## Data files

None.

## Notes

None.

## References

None.

## Warnings

None.

## Diagnostic Error Messages

None.

## Exit status

0 on successful completion.

## Known bugs

None.

## See also

Program name | Description
--- | ---
cpgplot | Plot CpG rich areas
cpgreport | Reports all CpG rich regions
newcpgreport | Report CpG rich areas
newcpgseek | Reports CpG rich regions

## Author(s)

This application was written by Richard Bruskiewich (rbsk@sanger.ac.uk) Informatics Division, The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.

## History

    Completed 18th June 1999.
    Last Modified 18th July 1999.

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments