| dbifasta

## Function

Index a fasta database

## Description

dbifasta indexes a flat file database of one or more files, and builds EMBL CD-ROM format index files. This format is used by the software on the EMBL database CD-ROM distribution and by the Staden package in addition to EMBOSS, and appears to be the most generally used and publicly available index file format for these databases.

Having created the EMBOSS indices for this file, a database can then be defined in the file emboss.defaults as something like:

    DB emrod [
       type: N
       format: fasta
       method: emblcd
       directory: /data/embl/fasta
    ]

### Fields Indexed

By default, dbifasta will index the ID name and the accession number (if present).
If they are present in your database, you may also specify that dbifasta should index the Sequence Version and GI number and the words in the description by using the '-fields' qualifier with the appropriate values.

## Usage

Here is a sample session with dbifasta

    % dbifasta
    Index a fasta database
        simple : >ID
         idacc : >ID ACC
         gcgid : >db:ID
      gcgidacc : >db:ID ACC
          dbid : >db ID
          ncbi : | formats
    ID line format [idacc]: idacc
    Database directory [.]: ../../data
    Wildcard database filename [*.dat]: emrod
    Database name: EMROD
    Release number [0.0]:
    Index date [00/00/00]:

Go to the output files for this example

## Command line arguments

       Standard (Mandatory) qualifiers:
       -idformat           menu       ID line format
       -directory          directory  Database directory
       -filenames          string     Wildcard database filename
      [-dbname]            string     Database name
       -release            string     Release number
       -date               string     Index date

       Additional (Optional) qualifiers: (none)
       Advanced (Unprompted) qualifiers:
       -fields             menu       Index fields
       -exclude            string     Wildcard filename(s) to exclude
       -indexdirectory     directory  Index directory
       -maxindex           integer    Maximum index length
       -sortoptions        string     Sort options, typically '-T .' to use
                                      current directory for work files and '-k
                                      1,1' to force GNU sort to use the first
                                      field
       -[no]systemsort     boolean    Use system sort utility
       -[no]cleanup        boolean    Clean up temporary files

       Associated qualifiers: (none)
       General qualifiers:
       -auto                boolean    Turn off prompts
       -stdout              boolean    Write standard output
       -filter              boolean    Read standard input, write standard output
       -options             boolean    Prompt for standard and additional values
       -debug               boolean    Write debug output to program.dbg
       -verbose             boolean    Report some/full command line options
       -help                boolean    Report command line options. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning             boolean    Report warnings
       -error               boolean    Report errors
       -fatal               boolean    Report fatal errors
       -die                 boolean    Report deaths

Standard (Mandatory) qualifiers | Allowed values | Default
--- | --- | ---

-idformat

ID line format

simple | (>ID)
idacc | (>ID ACC)
gcgid | (>db:ID)
gcgidacc | (>db:ID ACC)
dbid | (>db ID)
ncbi | (| formats)

idacc

-directory | Database directory | Directory | .
-filenames | Wildcard database filename | Any string is accepted | *.dat
[-dbname] (Parameter 1) | Database name | A string from 1 to 19 characters | Required
-release | Release number | A string up to 9 characters | 0.0
-date | Index date | Date string dd/mm/yy | 00/00/00
Additional (Optional) qualifiers | Allowed values | Default
(none)
Advanced (Unprompted) qualifiers | Allowed values | Default

-fields

Index fields

acnum | (Accession number)
seqvn | (Sequence Version and GI)
des | (Description)

acnum

-exclude | Wildcard filename(s) to exclude | Any string is accepted | An empty string is accepted
-indexdirectory | Index directory | Directory | .
-maxindex | Maximum index length | Integer 0 or more | 0
-sortoptions | Sort options, typically '-T .' to use current directory for work files and '-k 1,1' to force GNU sort to use the first field | Any string is accepted | -T . -k 1,1
-[no]systemsort | Use system sort utility | Boolean value Yes/No | Yes
-[no]cleanup | Clean up temporary files | Boolean value Yes/No | Yes

## Input file format

## Output file format

### Output files for usage example

### File: acnum.hit

This file contains non-printing characters and so cannot be displayed here.

### File: acnum.trg

This file contains non-printing characters and so cannot be displayed here.

### File: division.lkp

This file contains non-printing characters and so cannot be displayed here.

### File: entrynam.idx

This file contains non-printing characters and so cannot be displayed here.

dbifasta creates four index files. All are binary but with a simple format.

- division.lkp is the master index file, and has a 300 byte header containing the database name and date plus information on the record size. This header is followed by one record for each database file, giving the full file name for the data file, and optionally a second sequence file.
- entryname.idx is the entry name index. It has the same 300 byte header, mainly used to store the record size which will depend on the size of the longest entryname in the database. Each entry is stored in sorted alphanumeric order so that a binary search can be used to efficiently find any record. The record also holds the file number from division.lkp and the offsets in the data and sequence files for that entry.
- acnum.trg holds the accession number information. The file has the usual 300 byte header, and a sorted list of record by accession number. Each accession number record contains the first record number in acnum.hit and the total number of records in acnum.hit so that secondary (duplicated) accession numbers can be searched.
- acnum.hit is a very simple file. After the usual 300 byte header, each record simply holds the record number in entryname.idx. An accession number search will use acnum.trg to find a start position and number of records to read in this file, and will then simply read the entryname.idx records for each entry in turn.

## Data files

None.

## Notes

The indexing method depends on each entry having a unique entry name. No allowance is made for two entries with the same name so it is not possible to index EMBL and EMBLNEW together.

## References

None.

## Warnings

None.

## Diagnostic Error Messages

None.

## Exit status

It always exits with status 0.

## Known bugs

None.

## See also

Program name | Description
--- | ---
dbiblast | Index a BLAST database
dbiflat | Index a flat file database
dbigcg | Index a GCG formatted database

## Author(s)

Alan Bleasby (ableasby © rfcgr.mrc.ac.uk)
MRC Rosalind Franklin Centre for Genomics Research Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK

## History

Completed August 2000

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments