| extractfeat

## Function

Extract features from a sequence

## Description

extractfeat is a simple utility for extracting parts of a sequence that have been annotated as being a specific type of feature. These sub-sequences are writen to the output sequence file.

If the feature is annotated as being in the reverse sense of a nucleic acid sequence, then that feature's sub-sequence is reverse-complemented before being written out.

It is often useful to have some information on the context of the feature. extractfeat allows you to specify a number of bases or residues before and/or after the feature to write out.

If you are interested in extracting the sequence of the region around the start or end of the feature, then this can also be specified.

'joined' features can either be extracted as individual sequences, or as a single concatenated sequence if the '-join' qualifier is used.

Please remember that the output feature sequence is only as good as the annotation. If you rely upon other people's, or other program's annotation of features, then some of these will be incorrect.

## Usage

Here is a sample session with extractfeat

To write out the exons of a sequence:

    % extractfeat tembl:hsfau1 -type exon stdout
    Extract features from a sequence

    >HSFAU1_408_504 [exon] H.sapiens fau 1 gene
    cagtgacgtgacacgcagcccacggtctgtactgacgcgccctcgcttcttcctctttct
    cgactccatcttcgcggtagctgggaccgccgttcag
    >HSFAU1_774_856 [exon] H.sapiens fau 1 gene
    tcgccaatatgcagctctttgtccgcgcccaggagctacacaccttcgaggtgaccggcc
    aggaaacggtcgcccagatcaag
    >HSFAU1_951_1095 [exon] H.sapiens fau 1 gene
    gctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgctcctggcaggc
    gcgcccctggaggatgaggccactctgggccagtgcggggtggaggccctgactaccctg
    gaagtagcaggccgcatgcttggag
    >HSFAU1_1557_1612 [exon] H.sapiens fau 1 gene
    gtaaagtccatggttccctggcccgtgctggaaaagtgagaggtcagactcctaag
    >HSFAU1_1787_1912 [exon] H.sapiens fau 1 gene
    gtggccaaacaggagaagaagaagaagaagacaggtcgggctaagcggcggatgcagtac
    aaccggcgctttgtcaacgttgtgcccacctttggcaagaagaagggccccaatgccaac
    tcttaa

Go to the input files for this example

Example 2

To write out the exons with 10 extra bases at the start and end so that you can inspect the splice sites:

    % extractfeat tembl:hsfau1 -type exon -before 10 -after 10 stdout
    Extract features from a sequence

    >HSFAU1_408_504 [exon] H.sapiens fau 1 gene
    ggtcgctcagcagtgacgtgacacgcagcccacggtctgtactgacgcgccctcgcttct
    tcctctttctcgactccatcttcgcggtagctgggaccgccgttcaggtaagaatgg
    >HSFAU1_774_856 [exon] H.sapiens fau 1 gene
    ctttactcagtcgccaatatgcagctctttgtccgcgcccaggagctacacaccttcgag
    gtgaccggccaggaaacggtcgcccagatcaaggtaaggctgc
    >HSFAU1_951_1095 [exon] H.sapiens fau 1 gene
    ttccctgtaggctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgct
    cctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggaggccct
    gactaccctggaagtagcaggccgcatgcttggaggtgagtgaga
    >HSFAU1_1557_1612 [exon] H.sapiens fau 1 gene
    cccactacaggtaaagtccatggttccctggcccgtgctggaaaagtgagaggtcagact
    cctaaggtgagtgaga
    >HSFAU1_1787_1912 [exon] H.sapiens fau 1 gene
    ccttctccaggtggccaaacaggagaagaagaagaagaagacaggtcgggctaagcggcg
    gatgcagtacaaccggcgctttgtcaacgttgtgcccacctttggcaagaagaagggccc
    caatgccaactcttaagtcttttgta

Example 3

To write out the 10 bases around the start of all 'exon' features in the tembl database:

    % extractfeat tembl:*  -type exon -before 5 -after -5 stdout
    Extract features from a sequence

    >HSFAU1_408_504 [exon] H.sapiens fau 1 gene
    ctcagcagtg
    >HSFAU1_774_856 [exon] H.sapiens fau 1 gene
    ctcagtcgcc
    >HSFAU1_951_1095 [exon] H.sapiens fau 1 gene
    tgtaggctca
    >HSFAU1_1557_1612 [exon] H.sapiens fau 1 gene
    tacaggtaaa
    >HSFAU1_1787_1912 [exon] H.sapiens fau 1 gene
    tccaggtggc
    >HSFOS_889_1029 [exon] Human fos proto-oncogene (c-fos), complete cds.
    ccacgatgat
    >HSFOS_1783_2034 [exon] Human fos proto-oncogene (c-fos), complete cds.
    tctaggactt
    >HSFOS_2466_2573 [exon] Human fos proto-oncogene (c-fos), complete cds.
    tctagttatc
    >HSFOS_2688_3329 [exon] Human fos proto-oncogene (c-fos), complete cds.
    tacaggagac
    >HSTS1_1001_1205 [exon] Homo sapiens gene for thymidylate synthase, exons 1, 2, 3, 4, 5, 6, 7, complete cds.
    gcgccatgcc
    >HSTS1_2895_2968 [exon] Homo sapiens gene for thymidylate synthase, exons 1, 2, 3, 4, 5, 6, 7, complete cds.
    ttcagatgaa
    >HSTS1_5396_5570 [exon] Homo sapiens gene for thymidylate synthase, exons 1, 2, 3, 4, 5, 6, 7, complete cds.
    tccagggatc
    >HSTS1_11843_11944 [exon] Homo sapiens gene for thymidylate synthase, exons 1, 2, 3, 4, 5, 6, 7, complete cds.
    tacagattat
    >HSTS1_13449_13624 [exon] Homo sapiens gene for thymidylate synthase, exons 1, 2, 3, 4, 5, 6, 7, complete cds.
    ctcagatctt
    >HSTS1_14133_14204 [exon] Homo sapiens gene for thymidylate synthase, exons 1, 2, 3, 4, 5, 6, 7, complete cds.
    tatagccagg
    >HSTS1_15613_15750 [exon] Homo sapiens gene for thymidylate synthase, exons 1, 2, 3, 4, 5, 6, 7, complete cds.
    tttagcttca
    >AB009062_75_503 [exon] Homo sapiens HERG gene, exon 6.
    tgcaggtcct
    >HSFERG2_50_196 [exon] Human apoferritin H gene exons 2-4
    ttcagtctta
    >HSFERG2_453_578 [exon] Human apoferritin H gene exons 2-4
    ttcagaaacc
    >HSFERG2_674_999 [exon] Human apoferritin H gene exons 2-4
    tgcagttgtg
    >AP000504_13_134 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcactgtga
    >AP000504_868_930 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    gataccaaaa
    >AP000504_1081_1161 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cttaccaagc
    >AP000504_2752_2875 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cccacctctc
    >AP000504_3425_3584 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    gagacctcgg
    >AP000504_3818_4038 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    gttacccttt
    >AP000504_7507_7763 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ccagcccggg
    >AP000504_9766_9875 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ttcaggctgg
    >AP000504_10068_10193 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tccaggcgga
    >AP000504_10357_10463 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ttcaggtacc
    >AP000504_11631_11812 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cacagatctg
    >AP000504_13026_13434 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaggtggt
    >AP000504_14850_15164 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    gagggggagt
    >AP000504_15284_15383 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctaaggtcga
    >AP000504_15505_15578 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaggccgg
    >AP000504_15737_15856 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cctaggactt
    >AP000504_16337_16486 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tctaggcaat
    >AP000504_16676_16987 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgcaggcact
    >AP000504_18955_19059 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tcccttcaaa
    >AP000504_19185_19264 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cttaccggtt
    >AP000504_19402_19442 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaccaaat
    >AP000504_19797_19887 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcacctgtg
    >AP000504_20043_20390 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cctaccatgg
    >AP000504_20585_20645 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cttaccccca
    >AP000504_22296_22401 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    atccttgaaa
    >AP000504_23826_23936 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cccagctgac
    >AP000504_24719_25381 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cctaggtaag
    >AP000504_26111_26448 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    gctgtagagt
    >AP000504_28403_28525 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcacggttt
    >AP000504_28617_28671 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cttacccaag
    >AP000504_30215_30266 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tggccatggg
    >AP000504_31238_31363 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    aacaggtctc
    >AP000504_31486_31691 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctgagtgaaa
    >AP000504_33605_33675 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    gttcctcacc
    >AP000504_33846_34001 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcacctctg
    >AP000504_35893_36156 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tttacctgcc
    >AP000504_36240_36569 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcacctttg
    >AP000504_37069_37123 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cttacctgca
    >AP000504_40724_40877 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ggaagagcag
    >AP000504_41897_41953 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ggcaggatac
    >AP000504_42687_42753 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgcaggcttc
    >AP000504_42999_43085 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cccaggttac
    >AP000504_46996_47081 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    accaggcatt
    >AP000504_50596_50669 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgcagaggca
    >AP000504_50879_51001 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaggaagg
    >AP000504_52110_52224 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cacagctacc
    >AP000504_52348_52449 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcagtgtaa
    >AP000504_53426_53489 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cctaggtgat
    >AP000504_53901_53950 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tacagctgga
    >AP000504_54324_54447 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgcagggggt
    >AP000504_54909_55013 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    accagccacg
    >AP000504_55242_55305 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tctagggggc
    >AP000504_55723_55779 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgaagatacc
    >AP000504_55925_55987 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cccagggttc
    >AP000504_56128_56204 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cgtaggtatc
    >AP000504_56288_56386 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    accagcctca
    >AP000504_56484_56530 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgcaggggag
    >AP000504_56733_57055 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaggctcg
    >AP000504_59988_60040 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cagagatgca
    >AP000504_63714_63775 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cacagcagcc
    >AP000504_64760_64927 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tctaggtaag
    >AP000504_66908_67344 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cccaggcagc
    >AP000504_71741_72164 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    actgtggaat
    >AP000504_72744_73649 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tttaccataa
    >AP000504_73962_74192 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcatggcct
    >AP000504_74520_74709 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cctacctggg
    >AP000504_74856_74931 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaccaatg
    >AP000504_75374_75489 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaccatct
    >AP000504_76058_76160 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaccaggc
    >AP000504_77125_77207 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcacttcac
    >AP000504_77820_78148 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcaccggct
    >AP000504_79023_79187 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgattagaat
    >AP000504_79451_80175 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    agcaggtctc
    >AP000504_81318_81943 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgcaggtctc
    >AP000504_83295_85730 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ggctccccaa
    >AP000504_85819_85964 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    gagatgagga
    >AP000504_86305_86403 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cttaccttga
    >AP000504_86550_86648 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcactgtag
    >AP000504_86730_86803 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ccaaccttca
    >AP000504_87402_87556 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ctcacatgcg
    >AP000504_87948_88090 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    catacctcct
    >AP000504_91393_91628 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ccccgggcga
    >AP000504_92264_92384 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tttagagacc
    >AP000504_94413_94530 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ttcagatgaa
    >AP000504_94645_94841 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgcagatgtt
    >AP000504_95076_95129 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cccagagtga
    >AP000504_95289_95363 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    tgtagagtga
    >AP000504_96214_96449 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ttcagtgtcg
    >AP000504_97518_97647 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cacagccatc
    >AP000504_98437_98634 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cgcagcacga
    >AP000504_98843_99095 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    ttcagatcaa
    >AP000504_99439_99516 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cccagattct
    >AP000504_99847_99959 [exon] Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region, section 3/20.
    cacaggacag
    >AB000360_808_2266 [exon] Homo sapiens PIGC gene, complete cds.
    cttttagtaa
    >HSHBB_19289_19632 [exon] Human beta globin region on chromosome 11.
    ggagccaaca
    >HSHBB_19755_19977 [exon] Human beta globin region on chromosome 11.
    catagactcc
    >HSHBB_20833_21080 [exon] Human beta globin region on chromosome 11.
    aacagctcct
    >HSHBB_34478_34622 [exon] Human beta globin region on chromosome 11.
    tccacacact
    >HSHBB_34745_34967 [exon] Human beta globin region on chromosome 11.
    cacaggctcc
    >HSHBB_35854_36069 [exon] Human beta globin region on chromosome 11.
    aacagctcct
    >HSHBB_39414_39558 [exon] Human beta globin region on chromosome 11.
    tccacacact
    >HSHBB_39681_39903 [exon] Human beta globin region on chromosome 11.
    cacaggctcc
    >HSHBB_40770_40985 [exon] Human beta globin region on chromosome 11.
    aacagctcct
    >HSHBB_45710_45800 [exon] Human beta globin region on chromosome 11.
    acactgtagt
    >HSHBB_45922_46145 [exon] Human beta globin region on chromosome 11.
    cacagtctcc
    >HSHBB_46997_47124 [exon] Human beta globin region on chromosome 11.
    cccagctctt
    >HSHBB_54740_54881 [exon] Human beta globin region on chromosome 11.
    tgcttacact
    >HSHBB_55010_55232 [exon] Human beta globin region on chromosome 11.
    ctcagattac
    >HSHBB_56131_56389 [exon] Human beta globin region on chromosome 11.
    cgcagctctt
    >HSHBB_62137_62278 [exon] Human beta globin region on chromosome 11.
    tgcttacatt
    >HSHBB_62187_62278 [exon] Human beta globin region on chromosome 11.
    acaccatggt
    >HSHBB_62390_62408 [exon] Human beta globin region on chromosome 11.
    attggtctat
    >HSHBB_62409_62631 [exon] Human beta globin region on chromosome 11.
    cttaggctgc
    >HSHBB_63482_63742 [exon] Human beta globin region on chromosome 11.
    cacagctcct
    >GMGL01_363_460 [exon] Glycine max leghemoglobin gene or pseudogene (no mRNA detected).
    gaaatatggg
    >GMGL01_555_663 [exon] Glycine max leghemoglobin gene or pseudogene (no mRNA detected).
    aataggatat
    >GMGL01_2182_2286 [exon] Glycine max leghemoglobin gene or pseudogene (no mRNA detected).
    tgtaggtgcg
    >GMGL01_3065_3208 [exon] Glycine max leghemoglobin gene or pseudogene (no mRNA detected).
    cgtaggtggt

Example 4

To extract the CDS region with the exons joined into one sequence:

    % extractfeat tembl:hsfau1 -type CDS -join stdout
    Extract features from a sequence

    >HSFAU1_782_1912 [CDS] H.sapiens fau 1 gene
    atgcagctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacg
    gtcgcccagatcaaggctcatgtagcctcactggagggcattgccccggaagatcaagtc
    gtgctcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggag
    gccctgactaccctggaagtagcaggccgcatgcttggaggtaaagtccatggttccctg
    gcccgtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaag
    aagaagacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtg
    cccacctttggcaagaagaagggccccaatgccaactcttaa

Example 5

To write out the 7 residues around all phosphorylated residues in the tsw database:

    % extractfeat tsw:*  -type mod_res -value phosphorylation* -before 3 -after -4 stdout
    Extract features from a sequence

    >OPSD_HUMAN_343_343 [mod_res] RHODOPSIN.
    TETSQVA
    >PAXI_HUMAN_118_118 [mod_res] PAXILLIN.
    EHVYSFP

Go to the input files for this example

## Command line arguments

       Standard (Mandatory) qualifiers:
      [-sequence]          seqall     Sequence database USA
      [-outseq]            seqout     Output sequence USA

       Additional (Optional) qualifiers:
       -before             integer    If this value is greater than 0 then that
                                      number of bases or residues before the
                                      feature are included in the extracted
                                      sequence. This allows you to get the context
                                      of the feature. If this value is negative
                                      then the start of the extracted sequence
                                      will be this number of bases/residues before
                                      the end of the feature. So a value of '10'
                                      will start the extraction 10 bases/residues
                                      before the start of the sequence, and a
                                      value of '-10' will start the extraction 10
                                      bases/residues before the end of the
                                      feature. The output sequence will be padded
                                      with 'N' or 'X' characters if the sequence
                                      starts after the required start of the
                                      extraction.
       -after              integer    If this value is greater than 0 then that
                                      number of bases or residues after the
                                      feature are included in the extracted
                                      sequence. This allows you to get the context
                                      of the feature. If this value is negative
                                      then the end of the extracted sequence will
                                      be this number of bases/residues after the
                                      start of the feature. So a value of '10'
                                      will end the extraction 10 bases/residues
                                      after the end of the sequence, and a value
                                      of '-10' will end the extraction 10
                                      bases/residues after the start of the
                                      feature. The output sequence will be padded
                                      with 'N' or 'X' characters if the sequence
                                      ends before the required end of the
                                      extraction.
       -source             string     By default any feature source in the feature
                                      table is shown. You can set this to match
                                      any feature source you wish to show.
                                      The source name is usually either the name
                                      of the program that detected the feature or
                                      it is the feature table (eg: EMBL) that the
                                      feature came from.
                                      The source may be wildcarded by using '*'.
                                      If you wish to show more than one source,
                                      separate their names with the character '|',
                                      eg:
                                      gene* | embl
       -type               string     By default every feature in the feature
                                      table is extracted. You can set this to be
                                      any feature type you wish to extract.
                                      See http://www3.ebi.ac.uk/Services/WebFeat/
                                      for a list of the EMBL feature types and see
                                      Appendix A of the Swissprot user manual in
                                      http://www.expasy.ch/txt/userman.txt for a
                                      list of the Swissprot feature types.
                                      The type may be wildcarded by using '*'.
                                      If you wish to extract more than one type,
                                      separate their names with the character '|',
                                      eg:
                                      *UTR | intron
       -sense              integer    By default any feature type in the feature
                                      table is extracted. You can set this to
                                      match any feature sense you wish. 0 - any
                                      sense, 1 - forward sense, -1 - reverse sense
       -minscore           float      If this is greater than or equal to the
                                      maximum score, then any score is permitted
       -maxscore           float      If this is less than or equal to the maximum
                                      score, then any score is permitted
       -tag                string     Tags are the types of extra values that a
                                      feature may have. For example in the EMBL
                                      feature table, a 'CDS' type of feature may
                                      have the tags '/codon', '/codon_start',
                                      '/db_xref', '/EC_number', '/evidence',
                                      '/exception', '/function', '/gene',
                                      '/label', '/map', '/note', '/number',
                                      '/partial', '/product', '/protein_id',
                                      '/pseudo', '/standard_name', '/translation',
                                      '/transl_except', '/transl_table', or
                                      '/usedin'. Some of these tags also have
                                      values, for example '/gene' can have the
                                      value of the gene name.
                                      By default any feature tag in the feature
                                      table is extracted. You can set this to
                                      match any feature tag you wish to show.
                                      The tag may be wildcarded by using '*'.
                                      If you wish to extract more than one tag,
                                      separate their names with the character '|',
                                      eg:
                                      gene | label
       -value              string     Tag values are the values associated with a
                                      feature tag. Tags are the types of extra
                                      values that a feature may have. For example
                                      in the EMBL feature table, a 'CDS' type of
                                      feature may have the tags '/codon',
                                      '/codon_start', '/db_xref', '/EC_number',
                                      '/evidence', '/exception', '/function',
                                      '/gene', '/label', '/map', '/note',
                                      '/number', '/partial', '/product',
                                      '/protein_id', '/pseudo', '/standard_name',
                                      '/translation', '/transl_except',
                                      '/transl_table', or '/usedin'. Only some of
                                      these tags can have values, for example
                                      '/gene' can have the value of the gene name.
                                      By default any feature tag value in the
                                      feature table is shown. You can set this to
                                      match any feature tag valueyou wish to show.
                                      The tag value may be wildcarded by using
                                      '*'.
                                      If you wish to show more than one tag value,
                                      separate their names with a space or the
                                      character '|', eg:
                                      pax* | 10
       -join               boolean    Some features, such as CDS (coding sequence)
                                      and mRNA are composed of introns
                                      concatenated together. There may be other
                                      forms of 'joined' sequence, depending on the
                                      feature table. If this option is set TRUE,
                                      then any group of these features will be
                                      output as a single sequence. If the 'before'
                                      and 'after' qualifiers have been set, then
                                      only the sequence before the first feature
                                      and after the last feature are added.
       -featinname         boolean    To aid you in identifying the type of
                                      feature that has been output, the type of
                                      feature is added to the start of the
                                      description of the output sequence.
                                      Sometimes the description of a sequence is
                                      lost in subsequent processing of the
                                      sequences file, so it is useful for the type
                                      to be a part of the sequence ID name. If
                                      you set this to be TRUE then the name is
                                      added to the ID name of the output sequence.
       -describe           string     To aid you in identifying some further
                                      properties of a feature that has been
                                      output, this lets you specify one or more
                                      tag names that should be added to the output
                                      sequence Description text, together with
                                      their values (if any). For example, if this
                                      is set to be 'gene', then if any output
                                      feature has the tag (for example)
                                      '/gene=BRCA1' associated with it, then the
                                      text '(gene=BRCA1)' will be added to the
                                      Description line. Tags are the types of
                                      extra values that a feature may have. For
                                      example in the EMBL feature table, a 'CDS'
                                      type of feature may have the tags '/codon',
                                      '/codon_start', '/db_xref', '/EC_number',
                                      '/evidence', '/exception', '/function',
                                      '/gene', '/label', '/map', '/note',
                                      '/number', '/partial', '/product',
                                      '/protein_id', '/pseudo', '/standard_name',
                                      '/translation', '/transl_except',
                                      '/transl_table', or '/usedin'. Some of these
                                      tags also have values, for example '/gene'
                                      can have the value of the gene name.
                                      By default no feature tag is displayed. You
                                      can set this to match any feature tag you
                                      wish to show.
                                      The tag may be wildcarded by using '*'.
                                      If you wish to extract more than one tag,
                                      separate their names with the character '|',
                                      eg:
                                      gene | label

       Advanced (Unprompted) qualifiers: (none)
       Associated qualifiers:

       "-sequence" associated qualifiers
       -sbegin1             integer    Start of each sequence to be used
       -send1               integer    End of each sequence to be used
       -sreverse1           boolean    Reverse (if DNA)
       -sask1               boolean    Ask for begin/end/reverse
       -snucleotide1        boolean    Sequence is nucleotide
       -sprotein1           boolean    Sequence is protein
       -slower1             boolean    Make lower case
       -supper1             boolean    Make upper case
       -sformat1            string     Input sequence format
       -sdbname1            string     Database name
       -sid1                string     Entryname
       -ufo1                string     UFO features
       -fformat1            string     Features format
       -fopenfile1          string     Features file name

       "-outseq" associated qualifiers
       -osformat2           string     Output seq format
       -osextension2        string     File name extension
       -osname2             string     Base file name
       -osdirectory2        string     Output directory
       -osdbname2           string     Database name to add
       -ossingle2           boolean    Separate file for each entry
       -oufo2               string     UFO features
       -offormat2           string     Features format
       -ofname2             string     Features file name
       -ofdirectory2        string     Output directory

       General qualifiers:
       -auto                boolean    Turn off prompts
       -stdout              boolean    Write standard output
       -filter              boolean    Read standard input, write standard output
       -options             boolean    Prompt for standard and additional values
       -debug               boolean    Write debug output to program.dbg
       -verbose             boolean    Report some/full command line options
       -help                boolean    Report command line options. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning             boolean    Report warnings
       -error               boolean    Report errors
       -fatal               boolean    Report fatal errors
       -die                 boolean    Report deaths

Standard (Mandatory) qualifiers | Allowed values | Default
--- | --- | ---
[-sequence] (Parameter 1) | Sequence database USA | Readable sequence(s) | Required
[-outseq] (Parameter 2) | Output sequence USA | Writeable sequence | <sequence>.format
Additional (Optional) qualifiers | Allowed values | Default

-before

If this value is greater than 0 then that number of bases or residues before the feature are included in the extracted sequence. This allows you to get the context of the feature. If this value is negative then the start of the extracted sequence will be this number of bases/residues before the end of the feature. So a value of '10' will start the extraction 10 bases/residues before the start of the sequence, and a value of '-10' will start the extraction 10 bases/residues before the end of the feature. The output sequence will be padded with 'N' or 'X' characters if the sequence starts after the required start of the extraction.

Any integer value

0

-after

If this value is greater than 0 then that number of bases or residues after the feature are included in the extracted sequence. This allows you to get the context of the feature. If this value is negative then the end of the extracted sequence will be this number of bases/residues after the start of the feature. So a value of '10' will end the extraction 10 bases/residues after the end of the sequence, and a value of '-10' will end the extraction 10 bases/residues after the start of the feature. The output sequence will be padded with 'N' or 'X' characters if the sequence ends before the required end of the extraction.

Any integer value

0

-source

By default any feature source in the feature table is shown. You can set this to match any feature source you wish to show. The source name is usually either the name of the program that detected the feature or it is the feature table (eg: EMBL) that the feature came from. The source may be wildcarded by using '*'. If you wish to show more than one source, separate their names with the character '|', eg: gene* | embl

Any string is accepted

*

-type

By default every feature in the feature table is extracted. You can set this to be any feature type you wish to extract. See http://www3.ebi.ac.uk/Services/WebFeat/ for a list of the EMBL feature types and see Appendix A of the Swissprot user manual in http://www.expasy.ch/txt/userman.txt for a list of the Swissprot feature types. The type may be wildcarded by using '*'. If you wish to extract more than one type, separate their names with the character '|', eg: *UTR | intron

Any string is accepted

*

-sense | By default any feature type in the feature table is extracted. You can set this to match any feature sense you wish. 0 - any sense, 1 - forward sense, -1 - reverse sense | Any integer value | 0 - any sense, 1 - forward sense, -1 - reverse sense
-minscore | If this is greater than or equal to the maximum score, then any score is permitted | Any numeric value | 0.0
-maxscore | If this is less than or equal to the maximum score, then any score is permitted | Any numeric value | 0.0

-tag

Tags are the types of extra values that a feature may have. For example in the EMBL feature table, a 'CDS' type of feature may have the tags '/codon', '/codon_start', '/db_xref', '/EC_number', '/evidence', '/exception', '/function', '/gene', '/label', '/map', '/note', '/number', '/partial', '/product', '/protein_id', '/pseudo', '/standard_name', '/translation', '/transl_except', '/transl_table', or '/usedin'. Some of these tags also have values, for example '/gene' can have the value of the gene name. By default any feature tag in the feature table is extracted. You can set this to match any feature tag you wish to show. The tag may be wildcarded by using '*'. If you wish to extract more than one tag, separate their names with the character '|', eg: gene | label

Any string is accepted

*

-value

Tag values are the values associated with a feature tag. Tags are the types of extra values that a feature may have. For example in the EMBL feature table, a 'CDS' type of feature may have the tags '/codon', '/codon_start', '/db_xref', '/EC_number', '/evidence', '/exception', '/function', '/gene', '/label', '/map', '/note', '/number', '/partial', '/product', '/protein_id', '/pseudo', '/standard_name', '/translation', '/transl_except', '/transl_table', or '/usedin'. Only some of these tags can have values, for example '/gene' can have the value of the gene name. By default any feature tag value in the feature table is shown. You can set this to match any feature tag valueyou wish to show. The tag value may be wildcarded by using '*'. If you wish to show more than one tag value, separate their names with a space or the character '|', eg: pax* | 10

Any string is accepted

*

-join

Some features, such as CDS (coding sequence) and mRNA are composed of introns concatenated together. There may be other forms of 'joined' sequence, depending on the feature table. If this option is set TRUE, then any group of these features will be output as a single sequence. If the 'before' and 'after' qualifiers have been set, then only the sequence before the first feature and after the last feature are added.

Boolean value Yes/No

No

-featinname

To aid you in identifying the type of feature that has been output, the type of feature is added to the start of the description of the output sequence. Sometimes the description of a sequence is lost in subsequent processing of the sequences file, so it is useful for the type to be a part of the sequence ID name. If you set this to be TRUE then the name is added to the ID name of the output sequence.

Boolean value Yes/No

No

-describe

To aid you in identifying some further properties of a feature that has been output, this lets you specify one or more tag names that should be added to the output sequence Description text, together with their values (if any). For example, if this is set to be 'gene', then if any output feature has the tag (for example) '/gene=BRCA1' associated with it, then the text '(gene=BRCA1)' will be added to the Description line. Tags are the types of extra values that a feature may have. For example in the EMBL feature table, a 'CDS' type of feature may have the tags '/codon', '/codon_start', '/db_xref', '/EC_number', '/evidence', '/exception', '/function', '/gene', '/label', '/map', '/note', '/number', '/partial', '/product', '/protein_id', '/pseudo', '/standard_name', '/translation', '/transl_except', '/transl_table', or '/usedin'. Some of these tags also have values, for example '/gene' can have the value of the gene name. By default no feature tag is displayed. You can set this to match any feature tag you wish to show. The tag may be wildcarded by using '*'. If you wish to extract more than one tag, separate their names with the character '|', eg: gene | label

Any string is accepted

An empty string is accepted

Advanced (Unprompted) qualifiers | Allowed values | Default
(none)

## Input file format

extractfeat reads normal sequences with features.

Feature tables in Swissprot, EMBL, GFF, etc. format can be added using '-ufo featurefile' on the command line.

### Input files for usage example

'tembl:hsfau1' is a sequence entry in the example nucleic acid database 'tembl'

### Database entry: tembl:hsfau1

    ID   HSFAU1     standard; DNA; HUM; 2016 BP.
    XX
    AC   X65921; S45242;
    XX
    SV   X65921.1
    XX
    DT   13-MAY-1992 (Rel. 31, Created)
    DT   21-JUL-1993 (Rel. 36, Last updated, Version 5)
    XX
    DE   H.sapiens fau 1 gene
    XX
    KW   fau 1 gene.
    XX
    OS   Homo sapiens (human)
    OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
    OC   Eutheria; Primates; Catarrhini; Hominidae; Homo.
    XX
    RN   [1]
    RP   1-2016
    RA   Kas K.;
    RT   ;
    RL   Submitted (29-APR-1992) to the EMBL/GenBank/DDBJ databases.
    RL   K. Kas, University of Antwerp, Dept of Biochemistry T3.22,
    RL   Universiteitsplein 1, 2610 Wilrijk, BELGIUM
    XX
    RN   [2]
    RP   1-2016
    RX   MEDLINE; 92412144.
    RA   Kas K., Michiels L., Merregaert J.;
    RT   "Genomic structure and expression of the human fau gene: encoding the
    RT   ribosomal protein S30 fused to a ubiquitin-like protein.";
    RL   Biochem. Biophys. Res. Commun. 187:927-933(1992).
    XX
    DR   SWISS-PROT; P35544; UBIM_HUMAN.
    DR   SWISS-PROT; Q05472; RS30_HUMAN.
    XX
    FH   Key             Location/Qualifiers
    FH
    FT   source          1..2016
    FT                   /db_xref="taxon:9606"
    FT                   /organism="Homo sapiens"
    FT                   /clone_lib="CML cosmid"
    FT                   /clone="15.1"
    FT   mRNA            join(408..504,774..856,951..1095,1557..1612,1787..>1912)
    FT                   /gene="fau 1"
    FT   exon            408..504
    FT                   /number=1
    FT   intron          505..773
    FT                   /number=1
    FT   exon            774..856

      [Part of this file has been deleted for brevity]

    FT                   RAKRRMQYNRRFVNVVPTFGKKKGPNANS"
    FT   intron          857..950
    FT                   /number=2
    FT   exon            951..1095
    FT                   /number=3
    FT   intron          1096..1556
    FT                   /number=3
    FT   exon            1557..1612
    FT                   /number=4
    FT   intron          1613..1786
    FT                   /number=4
    FT   exon            1787..>1912
    FT                   /number=5
    FT   polyA_signal    1938..1943
    XX
    SQ   Sequence 2016 BP; 421 A; 562 C; 538 G; 495 T; 0 other;
         ctaccatttt ccctctcgat tctatatgta cactcgggac aagttctcct gatcgaaaac        60
         ggcaaaacta aggccccaag taggaatgcc ttagttttcg gggttaacaa tgattaacac       120
         tgagcctcac acccacgcga tgccctcagc tcctcgctca gcgctctcac caacagccgt       180
         agcccgcagc cccgctggac accggttctc catccccgca gcgtagcccg gaacatggta       240
         gctgccatct ttacctgcta cgccagcctt ctgtgcgcgc aactgtctgg tcccgccccg       300
         tcctgcgcga gctgctgccc aggcaggttc gccggtgcga gcgtaaaggg gcggagctag       360
         gactgccttg ggcggtacaa atagcaggga accgcgcggt cgctcagcag tgacgtgaca       420
         cgcagcccac ggtctgtact gacgcgccct cgcttcttcc tctttctcga ctccatcttc       480
         gcggtagctg ggaccgccgt tcaggtaaga atggggcctt ggctggatcc gaagggcttg       540
         tagcaggttg gctgcggggt cagaaggcgc ggggggaacc gaagaacggg gcctgctccg       600
         tggccctgct ccagtcccta tccgaactcc ttgggaggca ctggccttcc gcacgtgagc       660
         cgccgcgacc accatcccgt cgcgatcgtt tctggaccgc tttccactcc caaatctcct       720
         ttatcccaga gcatttcttg gcttctctta caagccgtct tttctttact cagtcgccaa       780
         tatgcagctc tttgtccgcg cccaggagct acacaccttc gaggtgaccg gccaggaaac       840
         ggtcgcccag atcaaggtaa ggctgcttgg tgcgccctgg gttccatttt cttgtgctct       900
         tcactctcgc ggcccgaggg aacgcttacg agccttatct ttccctgtag gctcatgtag       960
         cctcactgga gggcattgcc ccggaagatc aagtcgtgct cctggcaggc gcgcccctgg      1020
         aggatgaggc cactctgggc cagtgcgggg tggaggccct gactaccctg gaagtagcag      1080
         gccgcatgct tggaggtgag tgagagagga atgttctttg aagtaccggt aagcgtctag      1140
         tgagtgtggg gtgcatagtc ctgacagctg agtgtcacac ctatggtaat agagtacttc      1200
         tcactgtctt cagttcagag tgattcttcc tgtttacatc cctcatgttg aacacagacg      1260
         tccatgggag actgagccag agtgtagttg tatttcagtc acatcacgag atcctagtct      1320
         ggttatcagc ttccacacta aaaattaggt cagaccaggc cccaaagtgc tctataaatt      1380
         agaagctgga agatcctgaa atgaaactta agatttcaag gtcaaatatc tgcaactttg      1440
         ttctcattac ctattgggcg cagcttctct ttaaaggctt gaattgagaa aagaggggtt      1500
         ctgctgggtg gcaccttctt gctcttacct gctggtgcct tcctttccca ctacaggtaa      1560
         agtccatggt tccctggccc gtgctggaaa agtgagaggt cagactccta aggtgagtga      1620
         gagtattagt ggtcatggtg ttaggacttt ttttcctttc acagctaaac caagtccctg      1680
         ggctcttact cggtttgcct tctccctccc tggagatgag cctgagggaa gggatgctag      1740
         gtgtggaaga caggaaccag ggcctgatta accttccctt ctccaggtgg ccaaacagga      1800
         gaagaagaag aagaagacag gtcgggctaa gcggcggatg cagtacaacc ggcgctttgt      1860
         caacgttgtg cccacctttg gcaagaagaa gggccccaat gccaactctt aagtcttttg      1920
         taattctggc tttctctaat aaaaaagcca cttagttcag tcatcgcatt gtttcatctt      1980
         tacttgcaag gcctcaggga gaggtgtgct tctcgg                                2016
    //

### Input files for usage example 5

'tsw:*' is a sequence entry in the example protein database 'tsw'

## Output file format

The sequences of the specified features are written out.

The ID name of the sequence is formed from the original sequence name with the start and end positions of the feature appended to it. So if the feature came from a sequence with an ID name of 'XYZ' from positions 10 to 22, then the resulting ID name of the feature sequence will be 'XYZ_10_22'

The name of the type of feature is added to the start of the description of the sequence in brackets, e.g.: '[exon]'.

The sequence is written out as a normal sequence.

If the feature is in the reverse sense of a nucleic acid sequence, then it is reverse-complemented before being written.

## Data files

None.

## Notes

If a feature is specified as being a part of a different sequence entry in a database, then this feature is ignored.

If you are extracting 'joined' features and one of more of the component features is in a different sequence entry, then the whole joined feature is ignored.

## References

None.

## Warnings

None.

## Diagnostic Error Messages

If the end position of the sequence to be written is less than the start position, then the warning message "Extraction region end less than start for feature type [start-end] in ID name" is written and no sequence is output.

## Exit status

It always exits with status 0.

## Known bugs

None.

## See also

Program name | Description
--- | ---
biosed | Replace or delete sequence sections
codcopy | Reads and writes a codon usage table
coderet | Extract CDS, mRNA and translations from feature tables
cutseq | Removes a specified section from a sequence
degapseq | Removes gap characters from sequences
descseq | Alter the name or description of a sequence
entret | Reads and writes (returns) flatfile entries
extractseq | Extract regions from a sequence
listor | Write a list file of the logical OR of two sets of sequences
maskfeat | Mask off features of a sequence
maskseq | Mask off regions of a sequence
newseq | Type in a short new sequence
noreturn | Removes carriage return from ASCII files
notseq | Exclude a set of sequences and write out the remaining ones
nthseq | Writes one sequence from a multiple set of sequences
pasteseq | Insert one sequence into another
revseq | Reverse and complement a sequence
seqret | Reads and writes (returns) sequences
seqretsplit | Reads and writes (returns) sequences in individual files
showfeat | Show features of a sequence
skipseq | Reads and writes (returns) sequences, skipping first few
splitter | Split a sequence into (overlapping) smaller sequences
trimest | Trim poly-A tails off EST sequences
trimseq | Trim ambiguous bits off the ends of sequences
twofeat | Finds neighbouring pairs of features in sequences
union | Reads sequence fragments and builds one sequence
vectorstrip | Strips out DNA between a pair of vector sequences
yank | Reads a sequence range, appends the full USA to a list file

## Author(s)

Gary Williams (gwilliam © rfcgr.mrc.ac.uk)
MRC Rosalind Franklin Centre for Genomics Research Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK

## History

Written (Dec 12 2001) - Gary Williams

Added '-join' parameter (June 2002) - Gary Williams

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments

None