
                                 ehmmalign 



Function

   Align sequences to an HMM profile

Description

   EMBASSY HMMER is a suite of application wrappers to the original hmmer
   v2.3.2 applications written by Sean Eddy. hmmer v2.3.2 must be
   installed on the same system as EMBOSS and the location of the hmmer
   executables must be defined in your path for EMBASSY HMMER to work.

   Usage:
   ehmmalign [options] hmmfile seqfile outfile

   The outfile parameter is new to EMBASSY HMMER. The multiple sequence
   alignment is always written to outfile. The name of outfile is
   specified by the -o option as normal.

   hmmalign reads an HMM profile and a set of sequences , aligns the
   sequences to the profile HMM, and outputs a multiple sequence
   alignment . The set of sequences may be unaligned or aligned. If it is
   aligned, the existing alignment is ignored and hmmalign will align
   them the way it wants.

Algorithm

   Please read the Userguide.pdf distributed with the original HMMER and
   included in the EMBASSY HMMER distribution under the DOCS directory.

Usage

   Here is a sample session with ehmmalign


% ehmmalign ../ehmmcalibrate-ex-keep/globino.hmm globins630.fa globins630.ali 
Align sequences to an HMM profile

hmmalign - align sequences to an HMM profile
HMMER 2.3.2 (Oct 2003)
Copyright (C) 1992-2003 HHMI/Washington University School of Medicine
Freely distributed under the GNU General Public License (GPL)
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file:             ../ehmmcalibrate-ex-keep/globino.hmm
Sequence file:        ./ehmmalign-1234567890.1234
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Alignment saved in file globins630.ali

hmmalign  --informat FASTA --outformat A2M  -o globins630.ali ../ehmmcalibrate-
ex-keep/globino.hmm ./ehmmalign-1234567890.1234


   Go to the input files for this example
   Go to the output files for this example

Command line arguments

   Where possible, the same command-line qualifier names and parameter
   order is used as in the original hmmer. There are however several
   unavoidable differences and these are clearly documented in the
   "Notes" section below.

   More or less all options documented as "expert" in the original hmmer
   user guide are given in ACD as "advanced" options (-options must be
   specified on the command-line in order to be prompted for a value for
   them).

   Standard (Mandatory) qualifiers:
  [-hmmfile]           infile     File containing a HMM profile
  [-seqfile]           seqset     File containing a (set of) sequence(s)
  [-o]                 align      [*.ehmmalign] Multiple sequence alignment
                                  output file.

   Additional (Optional) qualifiers:
   -m                  boolean    [N] Include in the alignment only those
                                  symbols aligned to match states. Do not show
                                  symbols assigned to insert states.
   -q                  boolean    [N] Quiet; suppress all output except the
                                  alignment itself. Useful for piping or
                                  redirecting the output.

   Advanced (Unprompted) qualifiers:
   -mapali             infile     Reads an alignment from file and aligns it
                                  as a single object to the HMM; e.g. the
                                  alignment in file is held fixed. This allows
                                  you to align sequences to a model with
                                  hmmalign and view them in the context of an
                                  existing trusted multiple alignment. The
                                  alignment to the alignment is defined by a
                                  'map' kept in the HMM, and so is fast and
                                  guaranteed to be consistent with the way the
                                  HMM was constructed from the alignment. The
                                  alignment in the file must be exactly the
                                  alignment that the HMM was built from.
                                  Compare the -withali option.
   -withali            infile     Reads an alignment from file and aligns it
                                  as a single object to the HMM; e.g. the
                                  alignment in file is held fixed. This allows
                                  you to align sequences to a model with
                                  hmmalign and view them in the context of an
                                  existing trusted multiple alignment. The
                                  alignment to the alignment is done with a
                                  heuristic (nonoptimal) dynamic programming
                                  procedure, which may be somewhat slow and is
                                  not guaranteed to be completely consistent
                                  with the way the HMM was constructed (though
                                  it should be quite close). However, any
                                  alignment can be used, not just the
                                  alignment that the HMM was built from.
                                  Compare the -mapali option.

   Associated qualifiers:

   "-seqfile" associated qualifiers
   -sbegin2            integer    Start of each sequence to be used
   -send2              integer    End of each sequence to be used
   -sreverse2          boolean    Reverse (if DNA)
   -sask2              boolean    Ask for begin/end/reverse
   -snucleotide2       boolean    Sequence is nucleotide
   -sprotein2          boolean    Sequence is protein
   -slower2            boolean    Make lower case
   -supper2            boolean    Make upper case
   -sformat2           string     Input sequence format
   -sdbname2           string     Database name
   -sid2               string     Entryname
   -ufo2               string     UFO features
   -fformat2           string     Features format
   -fopenfile2         string     Features file name

   "-o" associated qualifiers
   -aformat3           string     Alignment format
   -aextension3        string     File name extension
   -adirectory3        string     Output directory
   -aname3             string     Base file name
   -awidth3            integer    Alignment width
   -aaccshow3          boolean    Show accession number in the header
   -adesshow3          boolean    Show description in the header
   -ausashow3          boolean    Show the full USA in the alignment
   -aglobal3           boolean    Show the full sequence in alignment

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write standard output
   -filter             boolean    Read standard input, write standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages

Input file format

  Alignment and sequence formats

   Input and output of alignments and sequences is limited to the formats
   that the original hmmer supports. These include stockholm, SELEX, MSF,
   Clustal, Phylip and A2M /aligned FASTA (alignments) and FASTA,
   GENBANK, EMBL, GCG, PIR (sequences). It would be fairly
   straightforward to adapt the code to support all EMBOSS-supported
   formats.

  Compressed input files

   Automatic processing of gzipped files is not supported.

   ehmmalign reads any normal sequence USAs.

  Input files for usage example

  File: ../ehmmcalibrate-ex-keep/globino.hmm

HMMER2.0  [2.3.2]
NAME  globins50
LENG  143
ALPH  Amino
RF    no
CS    no
MAP   yes
COM   hmmbuild -n globins50 --pbswitch 1000 --archpri 0.850000 --idlevel 0.6200
00 --swentry 0.500000 --swexit 0.500000 --wgsc -A -F globin.hmm ../../data/hmmn
ew/globins50.msf
COM   hmmcalibrate --mean 350.000000 --num 5000 --sd 350.000000 --seed 1 ../ehm
mbuild-ex-keep/globin.hmm
NSEQ  50
DATE  Sat Jul 15 12:00:00 2006
CKSUM 9858
XT      -8455     -4  -1000  -1000  -8455     -4  -8455     -4
NULT      -4  -8455
NULE     595  -1558     85    338   -294    453  -1158    197    249    902  -1
085   -142    -21   -313     45    531    201    384  -1998   -644
EVD   -35.959286   0.267496
HMM        A      C      D      E      F      G      H      I      K      L
  M      N      P      Q      R      S      T      V      W      Y
         m->m   m->i   m->d   i->m   i->i   d->m   d->d   b->m   m->e
         -450      *  -1900
     1    591  -1587    159   1351  -1874   -201    151  -1600    998  -1591
-693    389  -1272    595     42    -31     27   -693  -1797  -1134    14
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378   -450      *
     2   -926  -2616   2221   2269  -2845  -1178   -325  -2678   -300  -2596  -
1810    220  -1592    939   -974   -671   -939  -2204  -2785  -1925    15
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
     3   -638  -1715   -680    497  -2043  -1540     23  -1671   2380  -1641
-840   -222  -1595    437   1040   -564   -523  -1363   2124  -1313    16
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
     4    829  -1571    -37    660  -1856   -873    152  -1578    894  -1573
-678    769  -1273   1284     58    224    447  -1175  -1782  -1125    17
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
     5    369   -433   -475    286   -974  -1312    -19   -412    664    398
 406   1030  -1394    388   -214   -261     85   -166  -1227   -725    18
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
     6  -1291   -884  -3696  -3261  -1137  -3425  -2802   2322  -3066    111
  19  -3028  -3275  -2855  -3100  -2670  -1269   2738  -2450  -2062    19
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
     7    157   -413   -236    316  -1387  -1231     89   -863   1084   -431
-348    910  -1319    635    297     15    704   -483  -1497   -922    20
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
     8    770  -1431    -43    459  -1751   -340     78  -1449    440  -1497
-631    866  -1302    825    -51    953    364  -1076  -1750  -1121    21
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
     9    420   -186  -2172  -1577      8  -1818   -694   1477  -1281    760
 614  -1299  -1867  -1001  -1262   -189    -12   1401   -722   -364    22
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
    10   -961   -879  -2277  -1821   1366  -2213   -204   -399  -1500   -130
 -39  -1427  -2266  -1186  -1511   -159   -913   -367   4721   1177    23
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
    11    -48  -1782    809    844  -2073   1456      8  -1811    315  -1803
-932    180  -1365    921   -218    173   -115  -1399  -2018  -1327    24


  [Part of this file has been deleted for brevity]

     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   128   -415  -1926   1575   1399  -2219  -1163     17  -1983    527  -1929  -
1039    341  -1367   1597   -212    257   -222  -1536  -2109  -1387   144
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   129   -529  -1434   -629   -143  -1926   -626   -171  -1460   2679  -1597
-839   -309  -1599    207    317   -530   -510   -130  -1840  -1369   145
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   130    811   -397  -2389  -1807   1883  -2039   -907    594  -1512   1077
 687  -1532  -2065  -1201  -1483  -1125   -465   1067   -843   -472   146
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   131   -241   -102  -2327  -1710    724  -1767   -616    650  -1363   1074
1765   -718  -1809  -1026  -1252   -842   -181   1331   -541    695   147
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   132    723     95    385    823  -1820  -1168    167  -1540    875  -1362
-644    320  -1261    810    246    693    -67  -1141  -1753  -1098   148
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   133    551   -430  -1049   -481   -442    469   -241    465   -313    133
 947   -411  -1543    197   -587   -146    202    522   -843   -429   149
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   134  -1086   -777  -3351  -2800    816  -2898  -1861   1501  -2515   1149
 586  -2483  -2775  -2108  -2400  -2046  -1030   2380  -1511  -1216   150
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   135   1393   1409   -876   -345   -997   -525   -315   -590   -198   -847
-109   -420  -1441    -97    412    766   -130    139  -1306   -858   151
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   136     98  -1299     36    365  -1495  -1211   1241   -404    523   -952
-426   1174  -1303    511    -18    347    882   -853  -1566   -970   152
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   137   1308   -787    564   -132   -966  -1332   -203   -362    -49   -395
 -57   -305  -1481     49   -437   -190   -182   1020  -1282   -802   153
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   138  -1746  -1358  -3897  -3341   -216  -3621  -2478   1774  -3040   2442
1158  -3189  -3229  -2422  -2853  -2824  -1659    392  -1720  -1647   154
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   139   1176  -1289   -179    534  -1606   -607     34  -1278    734  -1372
-534     44  -1325    433    -89    521    826   -941  -1666  -1072   155
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6528  -7571   -894  -1115   -701  -1378      *      *
   140    602  -1500   -135    850  -1753  -1214   1951  -1452    838  -1484
 431    118  -1306    555    347    489   -153  -1085  -1723  -1092   156
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -22  -6602  -7644   -894  -1115   -701  -1378      *      *
   141    351  -1646   -165    546  -1976   -498     46  -1667   2193  -1662
-798     35  -1405    476    311    -73   -306  -1287  -1859  -1254   157
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -23  -6561  -7603   -894  -1115   -701  -1378      *      *
   142  -1995  -1606  -3095  -2870   1739  -3015    -98  -1012  -2520   -730
 655  -1990  -2962  -1884  -2326  -2167  -1915  -1128    548   4089   158
     -   -149   -500    233     43   -381    399    106   -626    210   -466
-720    275    394     45     96    359    117   -369   -294   -249
     -    -25  -6455  -7497   -894  -1115   -701  -1378      *      *
   143   -253  -1373   -267    301   -911   -565   1956   -450   1188  -1330
-497     33  -1352    502   1358   -205   -184   -941  -1604  -1026   159
     -      *      *      *      *      *      *      *      *      *      *
   *      *      *      *      *      *      *      *      *      *
     -      *      *      *      *      *      *      *      *      0
//

  File: globins630.fa

> BAHG_VITSP
MLDQQTINIIKATVPVLKEHGVTITTTFYKNLFAKHPEVRPLFDMGRQESLEQPKALAM
TVLAAAQNIENLPAILPAVKKIAVKHCQAGVAAAHYPIVGQELLGAIKEVLGDAATDDIL
DAWGKAYGVIADVfiqveadLYAQAVE
> GLB1_ANABR
PSVQGAAAQLTADVKKDLRDSWKVIGSDKKGNGVALMTTLFADNQETIGYFKRLGNVSQ
GMANDKLRGHSITLMYALQNFIDQLDNTDDLVCVVEKFAVNHITRKISAAEFGKINGPIK
KVLASKNFGDKYANAWAKLVAVVQAAL
> GLB1_ARTSX
ERVDPITGLSGLEKNAILDTWGKVRGNLQEVGKATFGKLFAAHPEYQQMFRFFQGVQLA
FLVQSPKFAAHTQRVVSALDQTLLALNRPSDQFVYMIKELGLDHINRGTDRSFVEYLKES
LGDSVDEFTVQSFGEVIVNFLNEGLRQA
> GLB1_CALSO
VSANDIKNVQDTWGKLYDQWDAVHAsKFYNKLFKDSEDISEAFVKAGTGSGIAMKRQAL
VFGAILQEFVANLNDPTALTLKIKGLCATHKTRGITNMELFAFALADLVAYMGTtISFTA
AQKASWTAVNDVILHQMSSYFATVA
> GLB1_CHITH
GPSGDQIAAAKASWNTVKNNQVDILYAVFKANPDIQTAFSQFAGKDLDSIKGTPDFSKH
AGRVVGLFSEVMDLLGNDANTPTILAKAKDFGKSHKSRASPAQLDNFRKSLVVYLKGATK
WDSAVESSWAPVLDFVFSTLKNEL
> GLB1_GLYDI
GLSAAQRQVIAATWKDIAGADNGAGVGKDCLIKFLSAHPQMAAVFGFSGASDPGVAALG
AKVLAQIGVAVSHLGDEGKMVAQMKAVGVRHKGYGNKHIKAQYFEPLGASLLSAMEHRIG
GKMNAAAKDAWAAAYADISGALISGLQS
> GLB1_LUMTE
ECLVTEGLKVKLQWASAFGHAHQRVAFGLELwkgILREHPEIKAPFSRVRGDNIYSPQF
GAHSQRVLSGLDITISMLDTPDmLAAQLAHLKVQHVERNLKPEFFDIFLKHLLHVLGDRL
GTHFDFGAWHDCVDQIIDGIKDI
> GLB1_MORMR
PIVDSGSVSPLSDAEKNKIRAAWDLVYKDYEKTGVDILVKFFTGTPAAQAFFPKFKGLT
TADDLKQSSDVRWHAERIINAVNDAVKSMDDTEKMSMKLKELSIKHAQSFYVDRQYFKVL
AGIIADTTAPGDAGFEKLMSMICILLSSAY
> GLB1_PARCH
GGTLAIQSHGDLTLAQKKIVRKTWHQLMRNKTSFVTDLFIRIFAYDPAAQNKFPQMAGM
SASQLRSSRQMQAHAIRVSSIMSEYIEELDSDILPELLATLARTHDLNKVGPAHYDLFAK
VLMEALQAELGSDFNQKTRDSWAKAFSIVQAVLLVKHG
> GLB1_PETMA
PIVDSGSVPALTAAEKATIRTAWAPVYAKYQSTGVDILIKFFTSNPAAQAFFPKFQGLT
SADQLKKSMDVRWHAERIINAVNDAVVAMDDTEKMSLKLRELSGKHAKSFQVDPQYFKVL
AAVIVDTVLPGDAGLEKLMSMICILLRSSY
> GLB1_PHESE
DCNTLKRFKVKHQWQQVFSGEhHRTEFSLHFWKEFLHDHPDLVSLFKRVQGENIYSPEF
QAHGIRVLAGLDSVIGVLDEDDTFTVQLAHLKAQHTERGTKPEYFDLFGTQLFDILGDKL
GTHFDQAAWRDCYAVIAAGIKP
> GLB1_SCAIN
PSVYDAAAQLTADVKKDLRDSWKVIGSDKKGNGVALMTTLFADNQETIGYFKRLGNVSQ
GMANDKLRGHSITLMYALQNFIDQLDNPDDLVCVVEKFAVNHITRKISAAEFGKINGPIK
KVLASKNFGDKYANAWAKLVAVVQAAL
> GLB1_TYLHE
TDCGILQRIKVKQQWAQVYSVGESRTDFAIDVFNNFFRTNPDRSLFNRVNGDNVYSPEF


  [Part of this file has been deleted for brevity]

GLSDAEWQLVLNVWGKVEADLAGHGQEVLIRLFHTHPETLEKFDKFKHLKSEDEMKASE
DLKKHGNTVLTALGAILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISEAIIHVLHSKH
PGDFGADAQAAMSKALELFRNDIAAQYKELGFQG
> MYG_ROUAE
GLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASE
DLKKHGATVLTALGGILKKKGQHEAQLKPLAQSHATKHKIPVKYLEFISEVIIQVLQSKH
PGDFGADAQGAMGKALELFRNDIAAKYKELGFQG
> MYG_SAISC
GLSDGEWQLVLNIWGKVEADIPSHGQEVLISLFKGHPETLEKFDKFKHLKSEDEMKASE
ELKKHGTTVLTALGGILKKKGQHEAELKPLAQSHATKHKIPVKYLELISDAIVHVLQKKH
PGDFGADAQGAMKKALELFRNDMAAKYKELGFQG
> MYG_SHEEP
GLSDGEWQLVLNAWGKVEADVAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASE
DLKKHGNTVLTALGGILKKKGHHEAEVKHLAESHANKHKIPVKYLEFISDAIIHVLHAKH
PSNFGADAQGAMSKALELFRNDMAAEYKVLGFQG
> MYG_SPAEH
GLSDGEWQLVLNVWGKVEGDLAGHGQEVLIKLFKNHPETLEKFDKFKHLKSEDEMKGSE
DLKKHGNTVLTALGGILKKKGQHAAEIQPLAQSHATKHKIPIKYLEFISEAIIQVLQSKH
PGDFGADAQGAMSKALELFRNDIAAKYKELGFQG
> MYG_TACAC
GLSDGEWQLVLKVWGKVETDITGHGQDVLIRLFKTHPETLEKFDKFKHLKTEDEMKASA
DLKKHGGVVLTALGSILKKKGQHEAELKPLAQSHATKHKISIKFLEFISEAIIHVLQSKH
SADFGADAQAAMGKALELFRNDMATKYKEFGFQG
> MYG_THUAL
ADFDAVLKCWGPVEADYTTMGGLVLTRLFKEHPETQKLFPKFAGIAQADIAGNAAISAH
GATVLKKLGELLKAKGSHAAILKPLANSHATKHKIPINNFKLISEVLVKVMHEKAGLDAG
GQTALRNVMGIIIADLEANYKELGFSG
> MYG_TUPGL
GLSDGEWQLVLNVWGKVEADVAGHGQEVLIRLFKGHPETLEKFDKFKHLKTEDEMKASE
DLKKHGNTVLSALGGILKKKGQHEAEIKPLAQSHATKHKIPVKYLEFISEAIIQVLQSKH
PGDFGADAQAAMSKALELFRNDIAAKYKELGFQG
> MYG_TURTR
GLSDGEWQLVLNVWGKVEADLAGHGQDVLIRLFKGHPETLEKFDKFKHLKTEADMKASE
DLKKHGNTVLTALGAILKKKGHHDAELKPLAQSHATKHKIPIKYLEFISEAIIHVLHSRH
PAEFGADAQGAMNKALELFRKDIAAKYKELGFHG
> MYG_VARVA
GLSDEEWKKVVDIWGKVEPDLPSHGQEVIIRMFQNHPETQDRFAKFKNLKTLDEMKNSE
DLKKHGTTVLTALGRILKQKGHHEAEIAPLAQTHANTHKIPIKYLEFICEVIVGVIAEKH
SADFGADSQEAMRKALELFRNDMASRYKELGFQG
> MYG_VULCH
GLSDGEWQLVLNIWGKVETDLAGHGQEVLIRLFKNHPETLDKFDKFKHLKTEDEMKGSE
DLKKHGNTVLTALGGILKKKGHHEAELKPLAQSHATKHKIPVKYLEFISDAIIQVLQSKH
SGDFHADTEAAMKKALELFRNDIAAKYKELGFQG
> MYG_ZALCA
GLSDGEWQLVLNIWGKVEADLVGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKRSE
DLKKHGKTVLTALGGILKKKGHHDAELKPLAQSHATKHKIPIKYLEFISEAIIHVLQSKH
PGDFGADTHAAMKKALELFRNDIAAKYRELGFQG
> MYG_ZIPCA
GLSEAEWQLVLHVWAKVEADLSGHGQEILIRLFKGHPETLEKFDKFKHLKSEAEMKASE
DLKKHGHTVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSRH
PSDFGADAQAAMTKALELFRKDIAAKYKELGFHG

Output file format

   ehmmalign outputs a graph to the specified graphics device. outputs a
   report format file. The default format is ...

  Output files for usage example

  File: globins630.ali

>BAHG_VITSP
.................mldqQTINIIKATV.PV...L.....K...E...H...GV.T.
....I...TTTFYKN..LFAKHPEVRPLFDMGRQESL-------..EQPKALAMTVLAAA
QN-IENLPA......ILPAVKKIAVKH..CQAG-..VAAAHYPIV.GQELLGAIKEV.L.
G.D.AATDDILDAWGKAYGVIADVFIQVEAdlyaqave
>GLB1_ANABR
.........psvqgaaaqltaDVKKDLRDSW.KV...I.....G...S...D...KK.G.
....N...GVALMTT..LFADNQETIGYFKRLGNVSQ---GMAN..DKLRGHSITLMYAL
QNFIDQLDNt...ddLVCVVEKFAVNH..ITR-K..ISAAEFGKI.NGPIKKVLAS-.-.
-.K.NFGDKYANAWAKLVAVVQAAL-----........
>GLB1_ARTSX
..........ervdpitglsgLEKNAILDTW.GK...V.....R...G...N...LQ.E.
....V...GKATFGK..LFAAHPEYQQMFRFFQGVQL-AFLVQS..PKFAAHTQRVVSAL
DQTLLALNRps..dqFVYMIKELGLDH..INRGT..-DRSFVEYL.KESL-----GD.S.
V.D.EFT------VQSFGEVIVNFLNEGLRqa......
>GLB1_CALSO
..................vsaNDIKNVQDTW.GK...L.....Y...D...Q...WD.A.
....V..hASKFYNK..LFKDSEDISEAFVKAGT---GSGIAMK..RQALVFGA-ILQEF
VANLNDPTA......LTLKIKGLCATH..KTRGI..TNMELFAFA.LADLVAYMGTT.I.
S.-.-FTAAQKASWTAVNDVILHQMSSYFAtva.....
>GLB1_CHITH
.................gpsgDQIAAAKASW.NT...V.....-...-...-...-K.N.
....N...QVDILYA..VFKANPDIQTAFSQFAG-KDLDSIKGT..PDFSKHAGRVVGLF
SEVMDLLGNdantptILAKAKDFGKSH..--KSR..ASPAQLDNF.RKSLVVYLKGA.-.
-.T.KWDSAVESSWAPVLDFVFSTLKNEL-........
>GLB1_GLYDI
.................glsaAQRQVIAATW.KD...I.....A...Ga.dN...GA.G.
....V...GKDCLIK..FLSAHPQMAAVFGFSGA--------SD..PGVAALGAKVLAQI
GVAVSHLGDe...gkMVAQMKAVGVRHkgYGNKH..IKAQYFEPL.GASLLSAMEHR.I.
G.G.KMNAAAKDAWAAAYADISGALISGLQs.......
>GLB1_LUMTE
.............eclvteglKVKLQWASAF.GH...A.....-...H...Q...RV.A.
....F...GLELWKG..ILREHPEIKAPFSRVRG-DN----IYS..PQFGAHSQRVLSGL
DITISMLDTp...dmLAAQLAHLKVQH..VER-N..LKPEFFDIF.LKHLLHVLGDR.L.
G.T.HFDF---GAWHDCVDQIIDGIKD--I........
>GLB1_MORMR
........pivdsgsvsplsdAEKNKIRAAW.DL...V.....Y...K...D...YE.K.
....T...GVDILVK..FFTGTPAAQAFFPKFKGLTTADDLKQS..SDVRWHAERIINAV
NDAVKSMDDt...ekMSMKLKELSIKH..AQSFY..VDRQYFKVL.AGII-------.-.
-.A.DTTAPGDAGFEKLMSMICILLSSAY-........
>GLB1_PARCH
.......ggtlaiqshgdltlAQKKIVRKTW.HQ...L.....M...R...N...KT.S.
....F...VTDLFIR..IFAYDPAAQNKFPQMAGMSA-SQLRSS..RQMQAHAIRVSSIM
SEYIEELDSd....iLPELLATLARTH..-DLNK..VGPAHYDLF.AKVLMEALQAE.L.
G.S.DFNQKTRDSWAKAFSIVQAVLLVKHG........
>GLB1_PETMA
........pivdsgsvpaltaAEKATIRTAW.AP...V.....Y...A...K...YQ.S.
....T...GVDILIK..FFTSNPAAQAFFPKFQGLTSADQLKKS..MDVRWHAERIINAV
NDAVVAMDDt...ekMSLKLRELSGKH..AKSFQ..VDPQYFKVL.AAVI-------.-.
-.V.DTVLPGDAGLEKLMSMICILLRSSY-........


  [Part of this file has been deleted for brevity]

P.G.DFGADAQGAMKKALELFRNDMAAKYKelgfqg..
>MYG_SHEEP
.................glsdGEWQLVLNAW.GK...V.....E...A...D...VA.G.
....H...GQEVLIR..LFTGHPETLEKFDKFKHLKTEAEMKAS..EDLKKHGNTVLTAL
GGILKKKGH......HEAEVKHLAESH..ANKHK..IPVKYLEFI.SDAIIHVLHAK.H.
P.S.NFGADAQGAMSKALELFRNDMAAEYKvlgfqg..
>MYG_SPAEH
.................glsdGEWQLVLNVW.GK...V.....E...G...D...LA.G.
....H...GQEVLIK..LFKNHPETLEKFDKFKHLKSEDEMKGS..EDLKKHGNTVLTAL
GGILKKKGQ......HAAEIQPLAQSH..ATKHK..IPIKYLEFI.SEAIIQVLQSK.H.
P.G.DFGADAQGAMSKALELFRNDIAAKYKelgfqg..
>MYG_TACAC
.................glsdGEWQLVLKVW.GK...V.....E...T...D...IT.G.
....H...GQDVLIR..LFKTHPETLEKFDKFKHLKTEDEMKAS..ADLKKHGGVVLTAL
GSILKKKGQ......HEAELKPLAQSH..ATKHK..ISIKFLEFI.SEAIIHVLQSK.H.
S.A.DFGADAQAAMGKALELFRNDMATKYKefgfqg..
>MYG_THUAL
.....................ADFDAVLKCW.GP...V.....E...A...D...YT.T.
....M...GGLVLTR..LFKEHPETQKLFPKFAGIAQ-ADIAGN..AAISAHGATVLKKL
GELLKAKGS......HAAILKPLANSH..ATKHK..IPINNFKLI.SEVLVKVMHEK.A.
G.-.-LDAGGQTALRNVMGIIIADLEANYKelgfsg..
>MYG_TUPGL
.................glsdGEWQLVLNVW.GK...V.....E...A...D...VA.G.
....H...GQEVLIR..LFKGHPETLEKFDKFKHLKTEDEMKAS..EDLKKHGNTVLSAL
GGILKKKGQ......HEAEIKPLAQSH..ATKHK..IPVKYLEFI.SEAIIQVLQSK.H.
P.G.DFGADAQAAMSKALELFRNDIAAKYKelgfqg..
>MYG_TURTR
.................glsdGEWQLVLNVW.GK...V.....E...A...D...LA.G.
....H...GQDVLIR..LFKGHPETLEKFDKFKHLKTEADMKAS..EDLKKHGNTVLTAL
GAILKKKGH......HDAELKPLAQSH..ATKHK..IPIKYLEFI.SEAIIHVLHSR.H.
P.A.EFGADAQGAMNKALELFRKDIAAKYKelgfhg..
>MYG_VARVA
.................glsdEEWKKVVDIW.GK...V.....E...P...D...LP.S.
....H...GQEVIIR..MFQNHPETQDRFAKFKNLKTLDEMKNS..EDLKKHGTTVLTAL
GRILKQKGH......HEAEIAPLAQTH..ANTHK..IPIKYLEFI.CEVIVGVIAEK.H.
S.A.DFGADSQEAMRKALELFRNDMASRYKelgfqg..
>MYG_VULCH
.................glsdGEWQLVLNIW.GK...V.....E...T...D...LA.G.
....H...GQEVLIR..LFKNHPETLDKFDKFKHLKTEDEMKGS..EDLKKHGNTVLTAL
GGILKKKGH......HEAELKPLAQSH..ATKHK..IPVKYLEFI.SDAIIQVLQSK.H.
S.G.DFHADTEAAMKKALELFRNDIAAKYKelgfqg..
>MYG_ZALCA
.................glsdGEWQLVLNIW.GK...V.....E...A...D...LV.G.
....H...GQEVLIR..LFKGHPETLEKFDKFKHLKSEDEMKRS..EDLKKHGKTVLTAL
GGILKKKGH......HDAELKPLAQSH..ATKHK..IPIKYLEFI.SEAIIHVLQSK.H.
P.G.DFGADTHAAMKKALELFRNDIAAKYRelgfqg..
>MYG_ZIPCA
.................glseAEWQLVLHVW.AK...V.....E...A...D...LS.G.
....H...GQEILIR..LFKGHPETLEKFDKFKHLKSEAEMKAS..EDLKKHGHTVLTAL
GGILKKKGH......HEAELKPLAQSH..ATKHK..IPIKYLEFI.SDAIIHVLHSR.H.
P.S.DFGADAQAAMTKALELFRKDIAAKYKelgfhg..

Data files

   None.

Notes

  1. Command-line arguments

   The following original HMMER options are not supported:
-h         : Use -help to get help information instead.
-informat  : All common sequence file formats are supported automatically.
-oneline   : Alignment format output is specified via the ACD file and /
             or the -aformat command line qualifier.
-outformat : Alignment format output is specified via the ACD file and / or
             the -aformat command line qualifier.

  2. Installing EMBASSY HMMER

   The EMBASSY HMMER package contains "wrapper" applications providing an
   EMBOSS-style interface to the applications in the original HMMER
   package version 2.3.2 developed by Sean Eddy. Please read the file
   INSTALL in the EMBASSY HMMER package distribution for installation
   instructions.

  3. Installing original HMMER

   To use EMBASSY HMMER, you will first need to download and install the
   original HMMER package. Please read the file 00README in the the
   original HMMER package distribution for installation instructions:
WWW home:       http://hmmer.wustl.edu/
Distribution:   ftp://ftp.genetics.wustl.edu/pub/eddy/hmmer/

  4. Setting up HMMER

   For the EMBASSY HMMER package to work, the directory containing the
   original HMMER executables *must* be in your path. For example if you
   executables were installed to "/usr/local/hmmer/bin", then type:
set path=(/usr/local/hmmer/bin/ $path)
rehash

  5. Getting help

   Please read the Userguide.pdf distributed with the original HMMER and
   included in the EMBASSY HMMER distribution under the DOCS directory.
   The first 3 chapters (Introduction, Installation and Tutorial) are
   particularly useful.

   Please read the 'Notes' section below for a description of the
   differences between the original and EMBASSY HMMER, particularly which
   application command line options are supported.

References

   None.

Warnings

  Types of input data

   hmmer v3.2.1 and therefore EMBASSY HMMER is only recommended for use
   with protein sequences. If you provide a non-protein sequence you will
   be reprompted for a protein sequence. To accept nucleic acid sequences
   you must replace instances of < type: "protein" > in the application
   ACD files with .

  Environment variables

   The original hmmer uses BLAST environment variables (below), if
   defined, to locate files. The EMBASSY HMMER does not.
BLASTDB   location of sequence databases to be searched
BLASMAT   location of substitution matrices
HMMERDB   location of HMMs

  Sequence input

   ehmmalign makes a temporary local copy of its input sequence data. You
   must ensure there is sufficient disk space for this in the directory
   that ehmmalign is run.

Diagnostic Error Messages

   None.

Exit status

   It always exits with status 0.

Known bugs

   None.

See also

   Program name                      Description
   ehmmbuild     Build a profile HMM from an alignment
   ehmmcalibrate Calibrate HMM search statistics
   ehmmconvert   Convert between profile HMM file formats
   ehmmemit      Generate sequences from a profile HMM
   ehmmfetch     Retrieve an HMM from an HMM database
   ehmmindex     Create a binary SSI index for an HMM database
   ehmmpfam      Search one or more sequences against an HMM database
   ehmmsearch    Search a sequence database with a profile HMM
   oalistat      Statistics for multiple alignment files
   ohmmalign     Align sequences with an HMM
   ohmmbuild     Build HMM
   ohmmcalibrate Calibrate a hidden Markov model
   ohmmconvert   Convert between HMM formats
   ohmmemit      Extract HMM sequences
   ohmmfetch     Extract HMM from a database
   ohmmindex     Index an HMM database
   ohmmpfam      Align single sequence with an HMM
   ohmmsearch    Search sequence database with an HMM

Author(s)

   This program is an EMBOSS conversion of a program written by Sean Eddy
   as part of his HMMER package.

   Although we take every care to ensure that the results of the EMBOSS
   version are identical to those from the original package, we recommend
   that you check your inputs give the same results in both versions
   before publication.

   Please report all bugs in the EMBOSS version to the EMBOSS bug team,
   not to the original author. Jon Ison (jison © rfcgr.mrc.ac.uk)
   MRC Rosalind Franklin Centre for Genomics Research Wellcome Trust
   Genome Campus, Hinxton, Cambridge, CB10 1SB, UK

   This program is an EMBASSY wrapper to a program written by Sean Eddy
   as part of his hmmer package.

   Please report any bugs to the EMBOSS bug team in the first instance,
   not to Sean Eddy.

History

Target users

   This program is intended to be used by everyone and everything, from
   naive users to embedded scripts.
