| enetphos

## Wiki

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## Function

Reports ser, thr and tyr phosphorylation sites in eukaryotic proteins

## Description

The NetPhos 2.0 server produces neural network predictions for serine, threonine and tyrosine phosphorylation sites in eukaryotic proteins.

## Usage

Here is a sample session with enetphos

    % enetphos
    Reports ser, thr and tyr phosphorylation sites in eukaryotic proteins
    Input (aligned) sequence set: EFTU_HUMAN.fsa
    Output file [eftu_human.enetphos]:

Go to the input files for this example
Go to the output files for this example

## Command line arguments

    Reports ser, thr and tyr phosphorylation sites in eukaryotic proteins
    Version: EMBOSS:6.2.0

       Standard (Mandatory) qualifiers:
      [-sequence]          seqset     (Aligned) sequence set filename and optional
                                      format, or reference (input USA)
      [-outfile]           outfile    [*.enetphos] Output file name

       Additional (Optional) qualifiers:
       -cutoff             float      [0.0] Report only scores above this value
                                      (Any numeric value)

       Advanced (Unprompted) qualifiers:
       -plot               boolean    [N] Produce graphics
       -two                boolean    [N] Run generic predictions only
       -kinase             boolean    [N] Run kinase-specific only
       -residue            menu       [all] Residues to predict with (Values: all
                                      (all); serine (serine); threonine
                                      (threonine); tyrosine (tyrosine))
       -gff                boolean    [N] Produce gff output
       -best               boolean    [N] Report only the highest score for each
                                      residue
       -addseq             boolean    [N] Add sequence to gff output

       Associated qualifiers:

       "-sequence" associated qualifiers
       -sbegin1            integer    Start of each sequence to be used
       -send1              integer    End of each sequence to be used
       -sreverse1          boolean    Reverse (if DNA)
       -sask1              boolean    Ask for begin/end/reverse
       -snucleotide1       boolean    Sequence is nucleotide
       -sprotein1          boolean    Sequence is protein
       -slower1            boolean    Make lower case
       -supper1            boolean    Make upper case
       -sformat1           string     Input sequence format
       -sdbname1           string     Database name
       -sid1               string     Entryname
       -ufo1               string     UFO features
       -fformat1           string     Features format
       -fopenfile1         string     Features file name

       "-outfile" associated qualifiers
       -odirectory2        string     Output directory

       General qualifiers:
       -auto               boolean    Turn off prompts
       -stdout             boolean    Write first file to standard output
       -filter             boolean    Read first file from standard input, write
                                      first file to standard output
       -options            boolean    Prompt for standard and additional values
       -debug              boolean    Write debug output to program.dbg
       -verbose            boolean    Report some/full command line options
       -help               boolean    Report command line options and exit. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning            boolean    Report warnings
       -error              boolean    Report errors
       -fatal              boolean    Report fatal errors
       -die                boolean    Report dying program messages
       -version            boolean    Report version number and exit

Qualifier | Type | Description | Allowed values | Default
--- | --- | --- | --- | ---
Standard (Mandatory) qualifiers
[-sequence] (Parameter 1) | seqset | (Aligned) sequence set filename and optional format, or reference (input USA) | Readable set of sequences | Required
[-outfile] (Parameter 2) | outfile | Output file name | Output file | <*>.enetphos
Additional (Optional) qualifiers
-cutoff | float | Report only scores above this value | Any numeric value | 0.0
Advanced (Unprompted) qualifiers
-plot | boolean | Produce graphics | Boolean value Yes/No | No
-two | boolean | Run generic predictions only | Boolean value Yes/No | No
-kinase | boolean | Run kinase-specific only | Boolean value Yes/No | No

-residue

list

Residues to predict with

all | (all)
serine | (serine)
threonine | (threonine)
tyrosine | (tyrosine)

all

-gff | boolean | Produce gff output | Boolean value Yes/No | No
-best | boolean | Report only the highest score for each residue | Boolean value Yes/No | No
-addseq | boolean | Add sequence to gff output | Boolean value Yes/No | No
Associated qualifiers
"-sequence" associated seqset qualifiers
-sbegin1 -sbegin_sequence | integer | Start of each sequence to be used | Any integer value | 0
-send1 -send_sequence | integer | End of each sequence to be used | Any integer value | 0
-sreverse1 -sreverse_sequence | boolean | Reverse (if DNA) | Boolean value Yes/No | N
-sask1 -sask_sequence | boolean | Ask for begin/end/reverse | Boolean value Yes/No | N
-snucleotide1 -snucleotide_sequence | boolean | Sequence is nucleotide | Boolean value Yes/No | N
-sprotein1 -sprotein_sequence | boolean | Sequence is protein | Boolean value Yes/No | N
-slower1 -slower_sequence | boolean | Make lower case | Boolean value Yes/No | N
-supper1 -supper_sequence | boolean | Make upper case | Boolean value Yes/No | N
-sformat1 -sformat_sequence | string | Input sequence format | Any string |
-sdbname1 -sdbname_sequence | string | Database name | Any string |
-sid1 -sid_sequence | string | Entryname | Any string |
-ufo1 -ufo_sequence | string | UFO features | Any string |
-fformat1 -fformat_sequence | string | Features format | Any string |
-fopenfile1 -fopenfile_sequence | string | Features file name | Any string |
"-outfile" associated outfile qualifiers
-odirectory2 -odirectory_outfile | string | Output directory | Any string |
General qualifiers
-auto | boolean | Turn off prompts | Boolean value Yes/No | N
-stdout | boolean | Write first file to standard output | Boolean value Yes/No | N
-filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N
-options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N
-debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N
-verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y
-help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N
-warning | boolean | Report warnings | Boolean value Yes/No | Y
-error | boolean | Report errors | Boolean value Yes/No | Y
-fatal | boolean | Report fatal errors | Boolean value Yes/No | Y
-die | boolean | Report dying program messages | Boolean value Yes/No | Y
-version | boolean | Report version number and exit | Boolean value Yes/No | N

## Input file format

enetphos reads any normal sequence USAs.

### Input files for usage example

### File: EFTU_HUMAN.fsa

    >EFTU_HUMAN  P49411 Elongation factor Tu, mitochondrial precursor (EF-Tu) (P43). - Homo sapiens (Human).
    MAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNV
    GTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAAR
    HYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYV
    NKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLL
    DAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIR
    TVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILS
    KEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQ
    RFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

## Output file format

### Output files for usage example

### File: eftu_human.enetphos

    >EFTU_HUMAN	452 amino acids
    #
    # netphos-3.1b prediction results
    #
    # Sequence		   # x   Context     Score   Kinase    Answer
    # -------------------------------------------------------------------
    # EFTU_HUMAN               5 T   MAAATLLRA   0.776   PKC        YES
    # EFTU_HUMAN               5 T   MAAATLLRA   0.520   cdc2       YES
    # EFTU_HUMAN               5 T   MAAATLLRA   0.442   CaM-II      .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.428   GSK3        .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.382   CKI         .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.350   DNAPK       .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.345   p38MAPK     .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.285   CKII        .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.226   ATM         .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.221   PKG         .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.204   RSK         .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.187   cdk5        .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.172   unsp        .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.125   PKA         .
    # EFTU_HUMAN               5 T   MAAATLLRA   0.073   PKB         .
    #
    # EFTU_HUMAN              10 T   LLRATPHFS   0.555   cdk5       YES
    # EFTU_HUMAN              10 T   LLRATPHFS   0.531   p38MAPK    YES
    # EFTU_HUMAN              10 T   LLRATPHFS   0.490   GSK3        .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.440   CaM-II      .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.369   CKI         .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.343   DNAPK       .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.341   PKG         .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.322   cdc2        .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.303   unsp        .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.275   RSK         .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.266   ATM         .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.249   CKII        .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.190   PKA         .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.099   PKC         .
    # EFTU_HUMAN              10 T   LLRATPHFS   0.093   PKB         .
    #
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.547   cdc2       YES
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.468   CaM-II      .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.444   GSK3        .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.371   CKI         .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.342   DNAPK       .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.332   CKII        .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.306   PKG         .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.304   PKC         .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.290   p38MAPK     .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.270   ATM         .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.248   RSK         .
    # EFTU_HUMAN              14 S   TPHFSGLAA   0.199   cdk5        .

      [Part of this file has been deleted for brevity]

    # EFTU_HUMAN             439 T   LVTNTLAMT   0.446   CKII        .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.441   CaM-II      .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.425   CKI         .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.424   GSK3        .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.420   cdc2        .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.364   PKA         .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.279   p38MAPK     .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.275   ATM         .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.264   PKG         .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.192   RSK         .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.160   cdk5        .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.160   unsp        .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.138   PKC         .
    # EFTU_HUMAN             439 T   LVTNTLAMT   0.078   PKB         .
    #
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.678   CKII       YES
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.480   CKI         .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.447   unsp        .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.443   GSK3        .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.425   CaM-II      .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.376   cdc2        .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.348   DNAPK       .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.315   PKG         .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.277   ATM         .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.251   p38MAPK     .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.249   RSK         .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.157   cdk5        .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.132   PKC         .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.081   PKB         .
    # EFTU_HUMAN             443 T   TLAMTEEEK   0.066   PKA         .
    #
        MAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKT   #     50
        YVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPE   #    100
        ERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILV   #    150
        VAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIR   #    200
        ELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVP   #    250
        ARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIR   #    300
        TVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQ   #    350
        KVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMP   #    400
        GEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIK   #    450
        WG                                                   #    500
    %1  ....T....T...S...................................T   #     50
    %1  Y....................T.T.................Y........   #    100
    %1  ......T........S................Y.......T.........   #    150
    %1  ..........T.................Y.........S...........   #    200
    %1  .......Y....T...............................TY....   #    250
    %1  ...............Y......T....T.................S....   #    300
    %1  ...T.......S................................S.....   #    350
    %1  .........S........................................   #    400
    %1  ......................T.........T.....T...T.......   #    450
    %1  ..

## Data files

None.

## Notes

None.

## References

None.

## Warnings

None.

## Diagnostic Error Messages

None.

## Exit status

It always exits with status 0.

## Known bugs

None.

## See also

Program name | Description
--- | ---
antigenic | Finds antigenic sites in proteins
digest | Reports on protein proteolytic enzyme or reagent cleavage sites
echlorop | Reports presence of chloroplast transit peptides
eiprscan | Motif detection
elipop | Prediction of lipoproteins
emast | Motif detection
ememe | Multiple EM for Motif Elicitation
ememetext | Multiple EM for Motif Elicitation. Text file only
enetnglyc | Reports N-glycosylation sites in human proteins
enetoglyc | Reports mucin type GalNAc O-glycosylation sites in mammalian proteins
epestfind | Finds PEST motifs as potential proteolytic cleavage sites
eprop | Reports propeptide cleavage sites in proteins
esignalp | Reports protein signal cleavage sites
etmhmm | Reports transmembrane helices
eyinoyang | Reports O-(beta)-GlcNAc attachment sites
fuzzpro | Search for patterns in protein sequences
fuzztran | Search for patterns in protein sequences (translated)
helixturnhelix | Identify nucleic acid-binding motifs in protein sequences
oddcomp | Identify proteins with specified sequence word composition
omeme | Motif detection
patmatdb | Searches protein sequences with a sequence motif
patmatmotifs | Scan a protein sequence with motifs from the PROSITE database
pepcoil | Predicts coiled coil regions in protein sequences
preg | Regular expression search of protein sequence(s)
pscan | Scans protein sequence(s) with fingerprints from the PRINTS database
sigcleave | Reports on signal cleavage sites in a protein sequence

## Author(s)

This program is an EMBOSS wrapper for a program written by the CBS group http://www.cbs.dtu.dk/index.shtml

The original CBS group application must be licensed and installed to use this wrapper.

Although we take every care to ensure that the results of the EMBOSS version are identical to those from the original package, we recommend that you check your inputs give the same results in both versions before publication.

Please report all bugs in the EMBOSS version to the EMBOSS bug team, not to the original author.

## History

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments

None