| mse

## Wiki

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## Function

Multiple Sequence Editor

## Description

This is a port of Will Gilbert's MSE multiple sequence editor from the original C code.

## Algorithm

## Usage

Here is a sample session with mse

    % mse dna.msf msf::mse.msf
    Multiple Sequence Editor

Go to the input files for this example
Go to the output files for this example

## Command line arguments

    Multiple Sequence Editor
    Version: EMBOSS:6.2.0

       Standard (Mandatory) qualifiers:
      [-sequence]          seqset     File containing a sequence alignment
      [-outseq]            seqoutset  [<sequence>.<format>] Sequence set filename
                                      and optional format (output USA)

       Additional (Optional) qualifiers: (none)
       Advanced (Unprompted) qualifiers:
       -gcgmode            boolean    [N] Use GCG Unix mode for keyboard shortcuts
                                      and commands
       -protein            boolean    [$(acdprotein)] Sequences are protein

       Associated qualifiers:

       "-sequence" associated qualifiers
       -sbegin1            integer    Start of each sequence to be used
       -send1              integer    End of each sequence to be used
       -sreverse1          boolean    Reverse (if DNA)
       -sask1              boolean    Ask for begin/end/reverse
       -snucleotide1       boolean    Sequence is nucleotide
       -sprotein1          boolean    Sequence is protein
       -slower1            boolean    Make lower case
       -supper1            boolean    Make upper case
       -sformat1           string     Input sequence format
       -sdbname1           string     Database name
       -sid1               string     Entryname
       -ufo1               string     UFO features
       -fformat1           string     Features format
       -fopenfile1         string     Features file name

       "-outseq" associated qualifiers
       -osformat2          string     Output seq format
       -osextension2       string     File name extension
       -osname2            string     Base file name
       -osdirectory2       string     Output directory
       -osdbname2          string     Database name to add
       -ossingle2          boolean    Separate file for each entry
       -oufo2              string     UFO features
       -offormat2          string     Features format
       -ofname2            string     Features file name
       -ofdirectory2       string     Output directory

       General qualifiers:
       -auto               boolean    Turn off prompts
       -stdout             boolean    Write first file to standard output
       -filter             boolean    Read first file from standard input, write
                                      first file to standard output
       -options            boolean    Prompt for standard and additional values
       -debug              boolean    Write debug output to program.dbg
       -verbose            boolean    Report some/full command line options
       -help               boolean    Report command line options and exit. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning            boolean    Report warnings
       -error              boolean    Report errors
       -fatal              boolean    Report fatal errors
       -die                boolean    Report dying program messages
       -version            boolean    Report version number and exit

Qualifier | Type | Description | Allowed values | Default
--- | --- | --- | --- | ---
Standard (Mandatory) qualifiers
[-sequence] (Parameter 1) | seqset | File containing a sequence alignment | Readable set of sequences | Required
[-outseq] (Parameter 2) | seqoutset | Sequence set filename and optional format (output USA) | Writeable sequences | <*>.format
Additional (Optional) qualifiers
(none)
Advanced (Unprompted) qualifiers
-gcgmode | boolean | Use GCG Unix mode for keyboard shortcuts and commands | Boolean value Yes/No | No
-protein | boolean | Sequences are protein | Boolean value Yes/No | $(acdprotein)
Associated qualifiers
"-sequence" associated seqset qualifiers
-sbegin1 -sbegin_sequence | integer | Start of each sequence to be used | Any integer value | 0
-send1 -send_sequence | integer | End of each sequence to be used | Any integer value | 0
-sreverse1 -sreverse_sequence | boolean | Reverse (if DNA) | Boolean value Yes/No | N
-sask1 -sask_sequence | boolean | Ask for begin/end/reverse | Boolean value Yes/No | N
-snucleotide1 -snucleotide_sequence | boolean | Sequence is nucleotide | Boolean value Yes/No | N
-sprotein1 -sprotein_sequence | boolean | Sequence is protein | Boolean value Yes/No | N
-slower1 -slower_sequence | boolean | Make lower case | Boolean value Yes/No | N
-supper1 -supper_sequence | boolean | Make upper case | Boolean value Yes/No | N
-sformat1 -sformat_sequence | string | Input sequence format | Any string |
-sdbname1 -sdbname_sequence | string | Database name | Any string |
-sid1 -sid_sequence | string | Entryname | Any string |
-ufo1 -ufo_sequence | string | UFO features | Any string |
-fformat1 -fformat_sequence | string | Features format | Any string |
-fopenfile1 -fopenfile_sequence | string | Features file name | Any string |
"-outseq" associated seqoutset qualifiers
-osformat2 -osformat_outseq | string | Output seq format | Any string |
-osextension2 -osextension_outseq | string | File name extension | Any string |
-osname2 -osname_outseq | string | Base file name | Any string |
-osdirectory2 -osdirectory_outseq | string | Output directory | Any string |
-osdbname2 -osdbname_outseq | string | Database name to add | Any string |
-ossingle2 -ossingle_outseq | boolean | Separate file for each entry | Boolean value Yes/No | N
-oufo2 -oufo_outseq | string | UFO features | Any string |
-offormat2 -offormat_outseq | string | Features format | Any string |
-ofname2 -ofname_outseq | string | Features file name | Any string |
-ofdirectory2 -ofdirectory_outseq | string | Output directory | Any string |
General qualifiers
-auto | boolean | Turn off prompts | Boolean value Yes/No | N
-stdout | boolean | Write first file to standard output | Boolean value Yes/No | N
-filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N
-options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N
-debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N
-verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y
-help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N
-warning | boolean | Report warnings | Boolean value Yes/No | Y
-error | boolean | Report errors | Boolean value Yes/No | Y
-fatal | boolean | Report fatal errors | Boolean value Yes/No | Y
-die | boolean | Report dying program messages | Boolean value Yes/No | Y
-version | boolean | Report version number and exit | Boolean value Yes/No | N

## Input file format

mse reads any normal sequence USAs.

### Input files for usage example

### File: dna.msf

    !!NA_MULTIPLE_ALIGNMENT

     dna.msf  MSF: 120  Type: N  January 01, 1776  12:00  Check: 3196 ..

     Name: MSFM1          Len:   120  Check:  8587  Weight:  1.00
     Name: MSFM2          Len:   120  Check:  6178  Weight:  1.00
     Name: MSFM3          Len:   120  Check:  8431  Weight:  1.00

    //

            MSFM1  ACGTACGTAC GTACGTACGT ACGTACGTAC GTACGTACGT ACGTACGTAC
            MSFM2  ACGTACGTAC GTACGTACGT ....ACGTAC GTACGTACGT ACGTACGTAC
            MSFM3  ACGTACGTAC GTACGTACGT ACGTACGTAC GTACGTACGT CGTACGTACG

            MSFM1  GTACGTACGT ACGTACGTAC GTACGTACGT ACGTACGTAC GTACGTACGT
            MSFM2  GTACGTACGT ACGTACGTAC GTACGTACGT ACGTACGTAC GTACGTACGT
            MSFM3  TACGTACGTA CGTACGTACG TACGTACGTA ACGTACGTAC GTACGTACGT

            MSFM1  ACGTACGTAC GTACGTACGT
            MSFM2  ACGTACGTTG CAACGTACGT
            MSFM3  ACGTACGTAC GTACGTACGT

## Output file format

mse outputs a graph to the specified graphics device. outputs a report format file. The default format is ...

### Output files for usage example

### File: mse.msf

    !!NA_MULTIPLE_ALIGNMENT 1.0

      mse.msf MSF: 120 Type: N 15/07/09 CompCheck: 2784 ..

      Name: MSFM1      Len: 120  Check: 8587 Weight: 1.00
      Name: MSFM2      Len: 120  Check: 5766 Weight: 1.00
      Name: MSFM3      Len: 120  Check: 8431 Weight: 1.00

    //

               1                                               50
    MSFM1      ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTAC
    MSFM2      ACGTACGTACGTACGTACGTacgt....ACGTACGTACGTACGTACGTAC
    MSFM3      ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTCGTACGTACG

               51                                             100
    MSFM1      GTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGT
    MSFM2      GTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGT
    MSFM3      TACGTACGTACGTACGTACGTACGTACGTAACGTACGTACGTACGTACGT

               101              120
    MSFM1      ACGTACGTACGTACGTACGT
    MSFM2      ACGTACGTTGCAACGTACGT
    MSFM3      ACGTACGTACGTACGTACGT

## Data files

None.

## Notes

None.

## References

None.

## Warnings

None.

## Diagnostic Error Messages

None.

## Exit status

It always exits with status 0.

## Known bugs

None.

## See also

Program name | Description
--- | ---
edialign | Local multiple alignment of sequences
emma | Multiple sequence alignment (ClustalW wrapper)
infoalign | Display basic information about a multiple sequence alignment
plotcon | Plot conservation of a sequence alignment
prettyplot | Draw a sequence alignment with pretty formatting
showalign | Display a multiple sequence alignment in pretty format
tranalign | Generate an alignment of nucleic coding regions from aligned proteins

## Author(s)

This program is an EMBOSS conversion of a program written by Will Gilbert as part of his MSE package.

## History

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.