                                  vrnalfold



Wiki

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   http://emboss.open-bio.org/wiki/Appdocs on the EMBOSS Wiki.

   Please help by correcting and extending the Wiki pages.

Function

   Calculate locally stable secondary structures of RNAs

Description

   This is a port of the Vienna RNA package program RNAlfold.

Algorithm

   See the original documentation for the Vienna RNA package
   http://www.tbi.univie.ac.at/~ivo/RNA/

Usage

   Here is a sample session with vrnalfold


% vrnalfold
Calculate locally stable secondary structures of RNAs
Input nucleotide sequence: tembl:d00596
Vienna RNALfold output file [d00596.vrnalfold]:


   Go to the input files for this example
   Go to the output files for this example

Command line arguments

Calculate locally stable secondary structures of RNAs
Version: EMBOSS:6.2.0

   Standard (Mandatory) qualifiers:
  [-sequence]          sequence   Nucleotide sequence filename and optional
                                  format, or reference (input USA)
  [-outfile]           outfile    [*.vrnalfold] Vienna RNALfold output file

   Additional (Optional) qualifiers: (none)
   Advanced (Unprompted) qualifiers:
   -constraintfile     infile     Vienna RNA structure constraints file
                                  (optional)
   -paramfile          infile     Vienna RNA parameters file (optional)
   -separation         integer    [150] Maximum allowed separation (Any
                                  integer value)
   -temperature        float      [37.0] Temperature (Any numeric value)
   -[no]gu             boolean    [Y] Allow GU pairs
   -[no]closegu        boolean    [Y] Allow GU pairs at end of helices
   -[no]lp             boolean    [Y] Allow lonely pairs
   -[no]convert        boolean    [Y] Convert T to U
   -nsbases            string     Non-standard bases (Any string)
   -[no]tetraloop      boolean    [Y] Stabilizing energies for tetra-loops
   -energy             menu       [0] Rarely used option to fold sequences
                                  from the ABCD... alphabet (Values: 0 (BP); 1
                                  (Any with GC); 2 (Any with AU parameters))
   -scale              float      [1.07] Estimate of ensemble free energy (Any
                                  numeric value)
   -dangles            menu       [1] Method (Values: 0 (Ignore); 1 (Only
                                  unpaired bases for just one dangling end); 2
                                  (Always use dangling energies); 3 (Allow
                                  coaxial stacking of adjacent helices))

   Associated qualifiers:

   "-sequence" associated qualifiers
   -sbegin1            integer    Start of the sequence to be used
   -send1              integer    End of the sequence to be used
   -sreverse1          boolean    Reverse (if DNA)
   -sask1              boolean    Ask for begin/end/reverse
   -snucleotide1       boolean    Sequence is nucleotide
   -sprotein1          boolean    Sequence is protein
   -slower1            boolean    Make lower case
   -supper1            boolean    Make upper case
   -sformat1           string     Input sequence format
   -sdbname1           string     Database name
   -sid1               string     Entryname
   -ufo1               string     UFO features
   -fformat1           string     Features format
   -fopenfile1         string     Features file name

   "-outfile" associated qualifiers
   -odirectory2        string     Output directory

   General qualifiers:
   -auto               boolean    Turn off prompts
   -stdout             boolean    Write first file to standard output
   -filter             boolean    Read first file from standard input, write
                                  first file to standard output
   -options            boolean    Prompt for standard and additional values
   -debug              boolean    Write debug output to program.dbg
   -verbose            boolean    Report some/full command line options
   -help               boolean    Report command line options and exit. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning            boolean    Report warnings
   -error              boolean    Report errors
   -fatal              boolean    Report fatal errors
   -die                boolean    Report dying program messages
   -version            boolean    Report version number and exit


Input file format

   vrnalfold reads any normal sequence USAs.

  Input files for usage example

   'tembl:d00596' is a sequence entry in the example nucleic acid database
   'tembl'

  Database entry: tembl:d00596

ID   D00596; SV 1; linear; genomic DNA; STD; HUM; 18596 BP.
XX
AC   D00596;
XX
DT   17-JUL-1991 (Rel. 28, Created)
DT   07-DEC-2007 (Rel. 94, Last updated, Version 6)
XX
DE   Homo sapiens gene for thymidylate synthase, complete cds.
XX
KW   .
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
XX
RN   [1]
RP   1-18596
RX   PUBMED; 2243092.
RA   Kaneda S., Nalbantoglu J., Takeishi K., Shimizu K., Gotoh O., Seno T.,
RA   Ayusawa D.;
RT   "Structural and functional analysis of the human thymidylate synthase
RT   gene";
RL   J. Biol. Chem. 265(33):20277-20284(1990).
XX
DR   GDB; 163670.
DR   GDB; 182340.
XX
CC   These data kindly submitted in computer readable form by:
CC   Sumiko Kaneda
CC   National Institute of Genetics
CC   1111 Yata
CC   Mishima 411
CC   Japan
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..18596
FT                   /organism="Homo sapiens"
FT                   /chromosome="18"
FT                   /map="18p11.32"
FT                   /mol_type="genomic DNA"
FT                   /clone="lambdaHTS-1 and lambdaHTS-3"
FT                   /db_xref="taxon:9606"
FT   repeat_region   1..148
FT                   /rpt_family="Alu"
FT   repeat_region   202..477
FT                   /rpt_family="Alu"
FT   prim_transcript 822..16246
FT                   /note="thymidylate synthase mRNA and introns"


  [Part of this file has been deleted for brevity]

     ttttgttttt agcttcagcg agaacccaga cctttcccaa agctcaggat tcttcgaaaa     15660
     gttgagaaaa ttgatgactt caaagctgaa gactttcaga ttgaagggta caatccgcat     15720
     ccaactatta aaatggaaat ggctgtttag ggtgctttca aaggagctcg aaggatattg     15780
     tcagtcttta ggggttgggc tggatgccga ggtaaaagtt ctttttgctc taaaagaaaa     15840
     aggaactagg tcaaaaatct gtccgtgacc tatcagttat taatttttaa ggatgttgcc     15900
     actggcaaat gtaactgtgc cagttctttc cataataaaa ggctttgagt taactcactg     15960
     agggtatctg acaatgctga ggttatgaac aaagtgagga gaatgaaatg tatgtgctct     16020
     tagcaaaaac atgtatgtgc atttcaatcc cacgtactta taaagaaggt tggtgaattt     16080
     cacaagctat ttttggaata tttttagaat attttaagaa tttcacaagc tattccctca     16140
     aatctgaggg agctgagtaa caccatcgat catgatgtag agtgtggtta tgaactttaa     16200
     agttatagtt gttttatatg ttgctataat aaagaagtgt tctgcattcg tccacgcttt     16260
     gttcattctg tactgccact tatctgctca gttccttcct aaaatagatt aaagaactct     16320
     ccttaagtaa acatgtgctg tattctggtt tggatgctac ttaaaagagt atattttaga     16380
     aataatagtg aatatatttt gccctatttt tctcatttta actgcatctt atcctcaaaa     16440
     tataatgacc atttaggata gagttttttt tttttttttt taaactttta taaccttaaa     16500
     gggttatttt aaaataatct atggactacc attttgccct cattagcttc agcatggtgt     16560
     gacttctcta ataatatgct tagattaagc aaggaaaaga tgcaaaacca cttcggggtt     16620
     aatcagtgaa atatttttcc cttcgttgca taccagatac ccccggtgtt gcacgactat     16680
     ttttattctg ctaatttatg acaagtgtta aacagaacaa ggaattattc caacaagtta     16740
     tgcaacatgt tgcttatttt caaattacag tttaatgtct aggtgccagc ccttgatata     16800
     gctatttttg taagaacatc ctcctggact ttgggttagt taaatctaaa cttatttaag     16860
     gattaagtag gataacgtgc attgatttgc taaaagaatc aagtaataat tacttagctg     16920
     attcctgagg gtggtatgac ttctagctga actcatcttg atcggtagga ttttttaaat     16980
     ccatttttgt aaaactattt ccaagaaatt ttaagccctt tcacttcaga aagaaaaaag     17040
     ttgttggggc tgagcactta attttcttga gcaggaagga gtttcttcca aacttcacca     17100
     tctggagact ggtgtttctt tacagattcc tccttcattt ctgttgagta gccgggatcc     17160
     tatcaaagac caaaaaaatg agtcctgtta acaaccacct ggaacaaaaa cagattttat     17220
     gcatttatgc tgctccaaga aatgctttta cgtctaagcc agaggcaatt aattaatttt     17280
     tttttttttg acatggagtc actgtccgtt gcccaggctg cagtgcagtg gcgcaatctt     17340
     ggctcactgc aacctccacc tcccaggttc aagtgattct cctgcctcag cctcccatgt     17400
     agctgggatc acaggcacct gccaccatgc ccggctaatt ttttgtattt tttgtagaga     17460
     cagggtttca ccatgttggc caggctggtc tcaaacacct gacctcaaat gatccacctg     17520
     cctcagcctc ccaaagtgtt gggattacag gcgtaagcca ccatgcccag ccctgaatta     17580
     atatttttaa aataagtttg gagactgttg gaaataatag ggcagaggaa catattttac     17640
     tggctacttg ccagagttag ttaactcatc aaactctttg ataatagttt gacctctgtt     17700
     ggtgaaaatg agccatgatc tcttgaacat gatcagaata aatgccccag ccacacaatt     17760
     gtagtccaaa ctttttaggt cactaacttg ctagatggtg ccaggttttt ttgcacaagg     17820
     agtgcaaatg ttaagatctc cactagtgag gaaaggctag tattacagaa gccttgtcag     17880
     aggcaattga acctccaagc cctggccctc aggcctgagg attttgatac agacaaactg     17940
     aagaaccgtt tgttagtgga tattgcaaac aaacaggagt caaagcttgg tgctccacag     18000
     tctagttcac gagacaggcg tggcagtggc tggcagcatc tcttctcaca ggggccctca     18060
     ggcacagctt accttgggag gcatgtagga agcccgctgg atcatcacgg gatacttgaa     18120
     atgctcatgc aggtggtcaa catactcaca caccctagga ggagggaatc agatcggggc     18180
     aatgatgcct gaagtcagat tattcacgtg gtgctaactt aaagcagaag gagcgagtac     18240
     cactcaattg acagtgttgg ccaaggctta gctgtgttac catgcgtttc taggcaagtc     18300
     cctaaacctc tgtgcctcag gtccttttct tctaaaatat agcaatgtga ggtggggact     18360
     ttgatgacat gaacacacga agtccctctg agaggttttg tggtgccctt taaaagggat     18420
     caattcagac tctgtaaata tccagaatta tttgggttcc tctggtcaaa agtcagatga     18480
     atagattaaa atcaccacat tttgtgatct atttttcaag aagcgtttgt attttttcat     18540
     atggctgcag cagctgccag gggcttgggg tttttttggc aggtagggtt gggagg         18596
//

Output file format

   vrnalfold outputs a graph to the specified graphics device. outputs a
   report format file. The default format is ...

  Output files for usage example

  File: d00596.vrnalfold

.((((..((.......))..)))). ( -3.80) 18572
.(((((((((((.....))..))))))))). (-11.00) 18553
.((((..(((((((((((.....))..))))))))).....)))). (-13.60) 18547
.((..((..((.((((...((((.((((....))))))))......)))).))....))..)). (-14.60) 18533
.(((.(..(((......)))..).))).....(((((((((((.....))..))))))))). (-15.60) 18522
.((..(((...(((.(..(((......)))..).))).....(((((((((((.....))..)))))))))......)))
..)). (-19.10) 18512
.(((.((((((............(((.(..(((......)))..).))).....(((((((((((.....))..))))))
)))...)))))).))). (-20.30) 18500
.(((.....)))......((..(((...(((.(..(((......)))..).))).....(((((((((((.....))..)
))))))))......)))..)). (-21.00) 18495
.(((((........)))))....((..(((...(((.(..(((......)))..).))).....(((((((((((.....
))..)))))))))......)))..)). (-22.00) 18490
.(((((((((((((......))))..)))))))))(..(((...(((.(..(((......)))..).))).....(((((
((((((.....))..)))))))))......)))..). (-23.20) 18479
.((.((((((((((((((......))))..)))))))))).)).....(((.(..(((......)))..).))).....(
((((((((((.....))..))))))))). (-24.50) 18475
.(((((.(((((.((((((((.....(((.....))).(((........)))....)))).)))).))))).))))).((
((..(((((((((((.....))..))))))))).....)))). (-26.20) 18470
.((..((.((.((.((((((((((((((......))))..)))))))))).)).....(((.(..(((......)))..)
.))).....(((((((((((.....))..)))))))))...)).))..)). (-26.90) 18465
.((((((.((((((((...((.((((((((((((((......))))..)))))))))).))...((((...((.......
.))..((((.((....)).))))...))))......)))))))).)).)))). (-31.50) 18457
.((..((..((((((((....(((..((.((((((((((((((......))))..)))))))))).)).....(((.(..
(((......)))..).)))....)))))))))))))..))...((((..((.......))..)))). (-32.80) 184
50
.(((((((....))))))) ( -4.60) 18439
.((((((......(((((((....))))))).((((((((....(((..((.((((((((((((((......))))..))
)))))))).)).....(((.(..(((......)))..).)))....)))))))))))...)))))) (-35.60) 1842
7
.((((.....)))). ( -4.70) 18405
.((((.((((.....)))))))). ( -7.50) 18400
.((((......((((.((((.....)))))))).......)))). (-12.42) 18390
.((((((..(((.(((........))))))....)))))). (-12.60) 18381
.(((((((((..(((.(((........))))))....))))))...))). (-13.80) 18378
.((((((...((((...))))...(((((((((((.....)))).((.(((((...((((.......(((((...((...
.))...)))))........))))))))).)).......))))))).)))))). (-34.36) 18375
.(((...(((((((..((((...)))))))))))...((((.....)))).))). (-14.60) 18369
((..(((...(((((((..((((...)))))))))))...((((.....)))).)))..)). (-15.00) 18366
.(((..(((...(((((((..((((...)))))))))))...((((.....)))).)))..))) (-15.20) 18364
.(((((.......((.(((((((..((((...))))))))))))).((((.....))))))))). (-15.30) 18360
.((((((((((.((.........)))))))))))). (-14.00) 18353
.((.((((((((((.((.........)))))))))))))). (-14.50) 18350
.((((....)))). ( -1.00) 18337
.(((((....((((....))))....((((((((((.((.........))))))))))))...))))). (-18.90) 1
8328
.((((((........((((....))))....((((((((((.((.........))))))))))))..)))))). (-19.
50) 18323
.(((((((((.........((((....))))....((((((((((.((.........))))))))))))..(((.(((..
......))))))..))))))))). (-28.60) 18319
.(((.((((.((((((.........((((....))))....((((((((((.((.........))))))))))))..)))
))).)))).))). (-27.40) 18313
.((((((((..((((((.((.((...............))..)).)))))).(((((((((.((.........)))))))
))))....)))))))). (-30.56) 18303
.((((((........(..((.((((.((((((.........((((....))))....((((((((((.((.........)
)))))))))))..)))))).)))).))..)........)))))). (-38.09) 18297
.(((((((...((((((((..((((((.((.((...............))..)).)))))).(((((((((.((......
...)))))))))))....))))))))..((((.((((.....)))))))).....)))).))). (-43.46) 18293
.(((.(....).))). ( -2.30) 18282
.((((((((.(....).))........((((((((..((((((.((.((...............))..)).)))))).((
(((((((.((.........)))))))))))....)))))))).)))))) (-36.56) 18277
.((((((..((((((((.......((((((((..(((((..(((..........................)))....)))
)))))))))).)))).)))))))))). (-28.87) 18272
((((((..((((......))))..)))))). ( -8.10) 18266
.((((..((((((..((((......))))..))))))........((((((((..((((((.((.((.............
..))..)).)))))).(((((((((.((.........)))))))))))....))))))))..)))). (-43.06) 182
59
.(((((...(((....)))..))))). (-10.40) 18250
.(((.....))). ( -2.80) 18234
.(((((((((.....))).....(((((...(((....)))..)))))..........)))))). (-17.10) 18228
.(((...((...(((.....))).....(((((...(((....)))..)))))....)).))). (-18.60) 18223
((..((..((((..(((...((...(((.....))).....(((((...(((....)))..)))))....)).)))....
.))))..))..)). (-24.00) 18210
.((((((((..(((..........)))..)))))))). (-13.60) 18207
.((((((........((((((((..(((..........)))..)))))))).......(((((...(((....)))..))
))).............))).))). (-28.40) 18193
.(((((((((.(...........((((((((..(((..........)))..)))))))).......(((((...(((...
.)))..)))))..........))))).))))). (-33.80) 18185


  [Part of this file has been deleted for brevity]

.(((...((....)).........((((....(((....))).....)))).))). (-14.80)  424
(((((((....((...((....))...))....))))))). ( -8.90)  415
.(((((.....(((((.......))))).((....)).......))))). ( -9.90)  402
.(((((.(.((((....)))).)......(((((..(((......................(((....)))...((....
.)))))..))))).......))))). (-33.70)  395
((((..(....)..)))). ( -5.10)  393
(((((.(.((...(((((.....(((((.......))))).((....)).......))))).....(((....)))..))
.).))))). (-28.50)  390
(((.((((.(((...((.(.((((....)))).).)).))).)))).))). (-19.80)  384
((.((((.(((.(((...((.(.((((....)))).).)).))).)))..((....)).........)))).)). (-22
.20)  381
.(((.((((.(((.(((...((.(.((((....)))).).)).))).)))..((....)).........)))).))) (-
22.40)  379
.(((((............(((.((((.(((...((.(.((((....)))).).)).))).)))).)))))))). (-29.
10)  366
.(((.((((((..(((((.((..(((.(((..(((.....)))))))))..))))))).)).)))).))). (-29.90)
  361
.((.(((.((((((..(((((.((..(((.(((..(((.....)))))))))..))))))).)).)))).))).)). (-
30.40)  358
.((((((((.....(((((............(((.((((.(((...((.(.((((....)))).).)).))).)))).))
))))))....)))))))). (-33.00)  353
(((((((..((.(((.((((((..(((((.((..(((.(((..(((.....)))))))))..))))))).)).)))).))
).)).....))))))). (-35.80)  350
.(((((((.(((((((..((.(((.((((((..(((((.((..(((.(((..(((.....)))))))))..))))))).)
).)))).))).)).....))))))).))))))). (-39.30)  341
.((((((..(.(((((((.(((((((..((.(((.((((((..(((((.((..(((.(((..(((.....))))))))).
.))))))).)).)))).))).)).....))))))).)))))))).)))))). (-50.30)  331
.((...((((((..(.(((((((.(((((((..((.(((.((((((..(((((.((..(((.(((..(((.....)))))
))))..))))))).)).)))).))).)).....))))))).)))))))).)))))).)). (-50.60)  326
.((.((...((((((..(.(((((((.(((((((..((.(((.((((((..(((((.((..(((.(((..(((.....))
)))))))..))))))).)).)))).))).)).....))))))).)))))))).)))))).)).)). (-51.70)  323
.(((.......))). ( -1.10)  297
.(((((...(((.......)))...))))). ( -5.80)  289
.((((.....(((((...(((.......)))...))))).....)))). ( -8.80)  280
.(((((.((((((.....))).....(((((...(((.......)))...)))))....)))))))). (-15.10)  2
64
.((..(((((......(((.....))).....(((((...(((.......)))...))))).)))))...)). (-16.2
0)  258
.((((.((..(((((......(((.....))).....(((((...(((.......)))...))))).)))))...)))))
). (-20.40)  253
.((((((.((....((((.((..(((((......(((.....))).....(((((...(((.......)))...))))).
)))))...)))))).)))))))) (-30.20)  240
((......)). ( -0.20)  228
(((((.......))))). ( -6.10)  219
(((((((.......))))).)). ( -8.00)  217
.((((...(((..........................(((((((.......))))).)).((((((..(((.(((.((..
((.(((((......(((.....)))...))))).))..)).)))....)))..))))))...)))..)))). (-36.40
)  180
.((....)). ( -2.60)  176
.(((((((.((....)).))))))). ( -5.20)  170
.((((((....(((((((.((....)).)))))))....)))))). ( -6.00)  160
.(((.((((((....(((((((.((....)).)))))))....)))))).))). ( -6.10)  156
.((((..(((.((((((....(((((((.((....)).)))))))....)))))).)))..........(((((......
.))))))))). (-19.00)  150
.(((((((((((((((((((((((((((((....(..........)....))))))))))))))))))))))))))))).
 (-41.90)  126
.((((((((((((((((((((((((((((((((((((....(..........)....)))))))))))))).........
...)))))))))))))))))))))). (-50.20)  119
.(((((((((((..((((((((((((((((((((((((((((((((((....(..........)....))))))))))))
))............))))))))))))))))))))))))))))))). (-64.10)  108
((.(((((((((((..((((((((((((((((((((((((((((((((((....(..........)....))))))))))
))))............))))))))))))))))))))))))))))))).)). (-68.00)  106
.((((((((((((.(((((((((((..((((((((((((((((((((((((((((((((((....(..........)...
.))))))))))))))............))))))))))))))))))))))))))))))).)))))))))))). (-93.50
)   95
.(((....))). ( -1.40)   81
.(((((((.((((....)).))))))))). (-15.50)   64
.((((..(((((((.((((....)).))))))))))))). (-21.80)   58
.((((((((..(((((((.((((....)).))))))))))))).)))) (-27.10)   54
.((((....)))). ( -2.30)   42
.((((..........((((....))))....((((..(((((((.((((....)).))))))))))))).)))) (-29.
90)   28
((((...)))). ( -3.60)   25
.(((.....((((...)))).......((((....))))..))). (-10.60)   16
.(((.(((((.(....).))))).))). (-13.30)    6
.((((.(((.(((((.(....).))))).))).)))). (-18.40)    1
CCUGUAGUCCCAGCUACGCGAGAGGCUGAGGCAGCAGAAUUACUUGAACCCAGGAGGCGGAGGUUGCAGUGAGCCGAGAU
CGCGCCACUGCACUCCAGCCUGGGUGAGAGAGCGAGACUCUGUCUCAAAAAAAAAAAAAAAAGACCGCCAGGGCUCAAAC
AAAAAACCUCGGAAAAGCCCUGGCGGUCUUUUUUUUUUUUUUUUUUUUUUUUUUUUGGGACAGUCUUGCUCUGUCGCCCA
GGCUGGAGUACAAUGGUCGGAUCUUGGCUCACUGCAACCUCUGCCUCCCAGGUUCAAGCAAUUCUUCUGCCUCAGCCUCC
CAAGUAGCCACCACGCCCAGCUAAUUUUUGUACUUUUAGUAGAGACGGGGGUUUCACCAUGUUGUCCAGGCUGGUCUUGA
ACUCCUGACCUCAGGUGAUCCACCCGCCUCGGCCCCCCAAAGUACUAGGAUUACAGGCGUGAGCCACCGCGUCCAGCGCC
CUGGCGGUUUUUAAUCAAGUAGAAAAGCUGCAUUAUACCACUUGCUUCGGUUGCUUCAGUGAGAACGAAGAAAUGGAAAU
GCAAAUCCCUUAUUAGUUGUAGGAAACAGAUCUCAAACAGCAGUUUUGUUGACAAGACCGCAGGAAAACGUGGGAACUGU
GCUGCUGGCUUAGAGAAGGCGCGGUCGACCAGACGGUUCCCAAAGGGCGCAGUCCUUCCCAGCCACCGCACCUGCAUCCA
GGUUCCCGGGUUUCCUAAGACUCUCAGCUGUGGCCCUGGGCUCCGUUCUGUGCCACACCCGUGGCUCCUGCGUUUCCCCC
UGGCGCACGCUCUCUAGAGCGGGGGCCGCCGCGACCCCGCCGAGCAGGAAGAGGCGGAGCGCGGGACGGCCGCGGGAAAA
GGCGCGCGGAAGGGGUCCUGCCACCGCGCCACUUGGCCUGCCUCCGUCCCGCCGCGCCACUUGGCCUGCCUCCGUCCCGC
CGCGCCACUUCGCCUGCCUCCGUCCCCCGCCCGCCGCGCCAUGCCUGUGGCCGGCUCGGAGCUGCCGCGCCGGCCCUUGC
CCCCCGCCGCACAGGAGCGGGACGCCGAGCCGCGUCCGCCGCACGGGGAGCUGCAGUACCUGGGGCAGAUCCAACACAUC
CUCCGCUGCGGCGUCAGGAAGGACGACCGCACGGGCACCGGCACCCUGUCGGUAUUCGGCAUGCAGGCGCGCUACAGCCU
GAGAGGUGACGCCGCGGGCCCCUGCGGGACGGGUGGCGGGAAGGAGGGAGGCGCGGCUGGGGAGAGCGCUCGGGAGCUGC
CGGGCGCUGCGGACCCCGUUUAGUCCUAACCUCAAUCCUGCCAGGGAGGGGACGCAUCGUCCUCCUCGCCUUACAGACGC
CGAAACGGAGGGUCCCAUUAGGGACGUGACUGGCGCGGGCAACACACACAGCAGCGACAGCCGGGAGGUAAGCCGCGUCC
CAGCGGCUCCGCGGCCGGGCUCGCAGUCGCCCCAGUGAUGCCGUGGCCCCCGAGGCGGGCGUCAUCGGGCAGCGUUUGCC
CAGUGCUGGAGGGUUAGGGAGAGCUGCCUGGGCUUGACCGCGCGCCGGUCUCAAAGUCCUGGCUUUGGCCCCUCCUCCGU
UUUCCCCUGUGGACCAUUCCGCUUCGCAGCGUUUUCAAAAACUGGAGCGAAAGUGAUGUGGGCGGGGCAAAGGCGGCGGG
AAGAGGACAGCACUGAAGCUGGCGCGGGAACUUGGUUUCCUGGUGGCCUCCCAUCCAAUCCCCACGAACCAGCUUUCCUC
UUAAACCUUGAAAAGAGAAAUUCGGGAGUUCGAGUUCUUAGUCGUCCUUUCCUCUUUCCUUUCCGACAGGAGCACCCCAG
GCAAAAAAUGUCUCGCGGGUCAUUGGCGCCAGGCUUUCAGGGGACAGUGGGGCGGGGCGGGGUGGGCACAGGACGUUAGG
CAGCCGUUGGCCCUCCCUAAGGCCACACCGUCCUGCCGUCCUGGAUCCUGCGCCAGCUGCGCGGGGGAGGGGACUCGAAG
GUGUGUGAGCCAGGGGCUGACCUUGACCGCUCAGAUAAAUGGAGCGCAGCCUUGACACAGGGGUGGAGGUGGUUUUGAAU
GGGGAAACCCAUUCGUGGUGAAGCAGAUUCACUGUAGCUAGCGGAAAAGCCCUCCGGCCCACGGACCCAUCUAGAGACGA
AUACAUAGCAGCUGCUGUGGCUGAUUGGCGUGGGACAGCGUGGGGAGUUUUGUCUGAGGAGAGGGAUCCACUUUUCUGCA
GCUCCAAGCCCAGGGGCCUUUGAUGAGCCAUAGACCUCAUUUUUAACCCACCUUUCUGCUUAGACAUUGAGCAAGUUACU
UCUCAUAUAGCUUCCCUAUAUGUUAAAAAUGGAGAAAAUAAUGCUUAGUAGGCAAUUCUGAUAAAAGCAGGUGCUUGCAA
AAAUCUCUCUGUUGUCUGAAUAUAAACUGUACCACAAGCGAGUGCGGAUGAACGAGGACUGCAUUUAAAGAUAAGUUUUU
ACACUUUCAUUUCUCUGUGGCUCGACACUUCUGAUGCCUCCCUUUUUGUUCCUGGGACACAUGCUUGGUGUUGUCUUCAC
ACCUUUGUGACAGGAUUAGCACUAGUGGGCAGUGGAUGAUAGCUCCUCCUCCCUUUUGCCACAUGUUCAUCCCUGCCCUC
GCCACCAUCUCACUGUGUGGAAUUCCUGUGUCCACUGGUCACCGGGGCACAGAAGUGCUGUCUCAGCCUGAAUCGGGCCA
CUGAUGGGACUUGCAGCCUGGGAGCUCCACCGUGAUCUCUGGCCCACUUUGCGGGAGUCUAGGCUUUCUGGAUGCUCCAG
GCCUCACGUCCCAGGGCAGUUUUCUUCCCUGAAGAAAGUUGGAUGGCAUGAUCUGUCUUCCCAUCUUGAAACCGUAUGGC
AAAUUGUUUUUCAGAUGAAUUCCCUCUGCUGACAACCAAACGUGUGUUCUGGAAGGGUGUUUUGGAGGAGUUGCUGUGGU
UUAUCAAGGUAAAGAAGUCGCUGCUAUUAGAAGUCAGUAGUCUGUUCUCAACACAGCAGCCAGUGAGAUCCUUUCAAAAC
UCAAAGCAGCCAGGUGUGGUGGCUCACGCCUGUAAUCCCACCGCUUUGGGAGGCUGAGUCAGAUCACCUGAGGUUAGGAA
UUUGGGACCAGCCUGGCCAACAUGGCGACACCCCAGUCUCUACUAAUAACACAAAAAAUUAGCCAGGUGUGCUGGUGCAU
GUCUGUAAUCCCAGCUACUCAGGAGGCUGAGGCAUGAGAAUUGCUCACGAGGCGGAGGUUGUAGUGAGCUGAGAUCGUGG
CACUGUACUCCAGCCUGGCGACAGAGGGAGAACCCAUGUCAAAAACAAAAAAAGACACCACCAAAGGUCAAAGCAUAUCA
UUCCUCACCCUCAAGCCCUUAGUGGCUCCAUUUCACUCAGUAAGAGCCACGGUCCUUAUGGUGUCCGUUUUUCAGCUCUG
ACCUUAGCUGCUGCUCUCUGCACCACCCUGCUGUUCUUGUGAGUUUUUGAGCACACCGGGACAUCCCCACUCCCUGGAAC
CUUCUUCCCCCACACUUGGCUUCUUCCUUUGAGUCUCUACUCCACUCGGGCAAGCCUUCCUAGACCUCCUGAUUUAAAAC
UGUGACUCUCCCCCAACCUCCUUGGUGUUUCUCCGUAGACGAACAUCACCAUCUGAUGUAUGUCAGCCUUUCCCUUCCCC
UGUUAGAAGGGGGACAGCAGGUAGUAAAAGUGAAAUGUGCUGUAAGCUUUAUGAGGGCAGAGGAUUUGUUUCUCGUGUUC
ACUGUUGUAUCGCCAGGGCCUCAAACACAGCCUGCCACAUAGUAGGAGUCAACAUAUAUUGAUCACUAAAUGUAGAUACC
ACCUGUGUUCCCAUGUUCAUAUAAAUUCUAGAAGAGUCUCUUCAGUAACAAGGUGAACCCCUUCCAGAGGGCUGAGUAGG
UACCUCAGGCCGGGGCCAGAGUGCUGUGAAGACAGCAGCAGCCCAGACCAAGCUUCUCUGUGUUCCGUGUCCUGGUCUAG
AACCAGCGAUGUUCUUUCUGACCAGUGCUUUUUGGAAGGUGGCUGAGGUCUGGGCUCAGGUCUGGGCCAUACUAGAAGCU
GGGAUCCCUUCUAUAGAGCACUUGGUAUGGCUUGUAUGGUCUUGGGGCAAGCCAGACCCAAGCCCUCUUAUCCCAUUUUA
GAAAGGGCUUCAAUUUGGAUCCAGCCCCAGGUCUGCCUUAGCUCUGUAUUCUUGGGGUAUUUUGUUCUGUAUUGGCCUAU
CUUGACUAACAAUGAGCCUUGGAUUUGAAACAUAUCAUCAGAAACCUCAGAAGACAACAUUCUUAAACUGGCUAGAGCCU
GGUCUGAAUGGAUGAAAAGGAGAGACUUUUGAAGCAAUAUGUAAAAGAUUGAGAAAUGAUUUGUUGGAAAUUUCUCAAUU
GGAGAAAUUUCUUUGAUUUGUUGGAAAUUUCUUUGAUUCUUUCUCAAUCAAAGAAAAUCGGGACAAACUCAACAAUAGAA
AGGGAGGAAGCAAGAUACUCAGAAAUAAAAUGCAUUCCCCUGUUUCAACUUAAUGCUUCAAUUCAGGAUUCUAAGGAAUC
CUUGCCAGGAAUGUCAGACUCACCUUGAUAGUUGGAGUUACUCCAUUGGUGACUCGAUCAAAUACAGGAGUUGAGGCACC
UGCACUGUAAAAUACUGAUUAGUCUGAUCAUUAGGAAUAUCCUGUAUGCCAGGUAGAAGAUACAUUGAACAGAUUGCAUG
UAGGCAUUAAAUUCAUUUUGGGGUAUUACAUAUAGACAACACAUUUCAUUAAGAAACAUAAAACUGUCAGAUCGGUGGAA
UACUUAAAAGCACUUGGAGGUGUUUAGCCUAAAAAGCUUAGUUGAGGGGAAUGGAAGAAAAGAUCUGGGAGGGUGGUUCC
AAAGAAGGGAUCAGACUAUCCUAAAGCCCUCAGGAAUCUGGGCUGGGACCACCUACUUAAAGAUAGGAUGGGCAGCUGGG
UGUGGUGGCUCACGCCUGUAAUCCCAGCACUUCGGGAGGCCGAAGCGGGCGGAUCACCUGAGGUCAGGAGUUCGAGGCCA
GCCUGACCAACAUGGAGAAACGCUGUCUCUACUAAAAAUACAAAAUUAGCUGGGUGUAGUGGCGCAUGCCUGUAAUCCCA
GCUACUCGGGAGGCUGAGGCAGGGGAAUCGCUUGAACCUGGGAGGUGGAGGGUGCCGUGAGCCACGAUCGCGCCAUUGCA
CUCCAGCCUGGGCAACAAGAGCGAAACUCUCAAAAAACAAAAAAAAGGAUGGGUUCCAUAUGGGUGGUGUCAAGUGCCCA
CCUCCUAGCAAGUCAGCAGGGGCCAGAGGCCCUUGUAAGUGGUGUCUCGGGGGGAUCAACUGAGAUGGCUUAAGAUUUAC
CUGGAUGCCUGCUCUGCUCUCCCCAUCUCUUCCAGGGAUCCACAAAUGCUAAAGAGCUGUCUUCCAAGGGAGUGAAAAUC
UGGGAUGCCAAUGGAUCCCGAGACUUUUUGGACAGCCUGGGAUUCUCCACCAGAGAAGAAGGGGACUUGGGCCCAGUUUA
UGGCUUCCAGUGGAGGCAUUUUGGGGCAGAAUACAGAGAUAUGGAAUCAGGUGAGGAGAUAGAACAAUGCCUUCCAUUUC
CGGGUGCCCUUCCUAGCACGUGUUUGCUCCGUUGUUUUAGAUAAGGUCUGGGGGAUGAGUCAAUGUCACAGGAGCUGAUG
UAUAGCUUUGACCUUGUGAGGGGUGGUGCCAGGUUGAAGCCACAAUUAACGCCUACUGAAGGCCGUUUCACAUCUUUUUU
UUUUUUUUUUUUUUAAUUAUUAUACUUUAAGUUUUAGGGUACAUGUGCACAAUGUGCAGGUUAGUUACAUAUGUAUACAU
GUGCCAUGCUGGUGCGCUGCACCACUAACUCACCAUCUAGCAUCAGGUAUAUCUCCCAAUGCUAUCCCUCCCCCCUCCUC
CCACCCCACAACAUCCCCAGAGUGUGAUGUUCCCCUUCCUGUGUCCAUAUGUUCUCGUUGUUCGAUUCCCACUAUGAGUG
AGAAUAUGCGGUGUUUGGUUUUUUGUUCUUGCGAUAGUUUACUGAGAAUGAUGAUUUCCAUUUCACCACGUCCCUACAGA
GGACAUGAACUCAUCAUUUUUUAUGGCUGCAUAGUAUUCCAUGGUGUAUAUGUGCCACAUUUUCUUAAUCCAGUCUAUCA
UGUUGGACAUUUGGGUUGGUUCCAAGUCUUUGCCUAUUGUGAAUAGUGCCACAAUAAACAUACGUGUGCAUGUGUCUUUA
UAGCAGCAUGAUUUAAUAGUCCUUUGGGUAUAUACCCAGUAAUGGGAUGGCUGGGUCAAAUGGUAUUUCUAGUUCUAGAU
CCCCGAGGAAUCGCCACACUGACUUCCACAAUGGUUGAACUAGUUUACAGUCCCACCAACAGUGUCAAAGUGUCCUAUUU
CUCCACAUCCUCUCCAGCACCUGUUGUUUCCUGACUUUUUAAUGAUUGCCAUUCUAACUGGUGUGAGAUGGUAUCUCAUU
GUGGUUUUGAUUUGCGUUUCUCUGAUGGCCAGUGAUGGUGAGCAUUUUUUCAUGUGUUUUUUGGCUGCAUAAAUGUCUUC
UUUUGAGAAGUGUCUGUUCAUGUCCUUCGCCCACUUUUUGAUGGGGUUGUUUUUUUCUUAUAAAUUUGUUUGAGUUCAUU
GUAGAUUCUGGAUAUUAGCCCUUUGUCAGAUGAGUAGGUUGCAAAAAUGUUCUCCCAUUUUGUGGGUUGCCUGUUCACUC
UGAUGGUAGUUUCUUUUGCUGUGCAGAAGCUCUUUAGUUUAAUUAGAUCCCAUUUGUCAAUUUUGGCUUUUGUUGCCAUU
GCUUUUGGCAUAGGCAUGAAGUCCUUGCCCAUGCCUAUGUCCUGAAUGGUAAUGCCUAGGUUUUCUUCUAGGGUUUUUAU
GGUUUUAGGUCUAACGUUUAAGUCUUUAAUCCAUCUUGAAUUGAUUUUUGUAUAAGGUGUAAGGAAGGGAUCCAGUUUCA
GCUUUUUACAUAUGGCUAGCCAGUUUUCCCAGCACCAUUUAUUACAUAGGGAAUCCUUUCCCCAUUGCUUGUUUUUCUCA
GGUUUGUCAAAGAUCAGAUAGUUGUAGAUAUGCGGCGUUAUUUCUGAGGGCUCUGUUCUGUUCCAUUGAUCUAUGUGUCU
GUUUUGGUACCAGUACCAUACUGUUUUGGUUACUGUAGCCUUGUAGUAUAGUUUGAAGUCAGGUAGCGUGAUGCCUCCAG
CUUUGUUCUUUUGGCUUAGGAUUGACUUGGCGAUGCGGGCUCUUUUUUGGUUCCAUAUGAACUUUAAAGUAGUUUUUUCC
AAUUCUGUGAAGAAAGUCAUUGGUAGCUUGAUGGGGAUGGCAUUGAAUCUAUAAAUUACCUUGGGCAGUAUGGCCAUUUU
CACGAUAUUGAUUCUUCCUACCCAUGAGCAUGGAAUGGUCUUCCAUUUCUUUGUAUCCUCUUUUAUUUCAUUGAGCAGUG
GUUUGUAGUUCUCCUUGAAGAGGUCCUUCACAUCCCUUUUAAGGUGGAUUCCUAGGUAUUUUAUUCUCUUUGAAGCAAUU
GUGAGUGGAAGUUCACUCAUGAUUUGGCUCUCUGUUUGUCUGUUAUUGGUGUAUAAGAAUGCUUGUGAUUUUUGCAGAUU
GAUUUUAUAUCCUGAGACUUUGCUGAAGCUGCUUAUCAGCUUAAGGAGAUUUUGGGCUGAGACAAUGGGGUUUUCUAGAU
AUACAAUCAUGUCGUCUGCAAACAGGGACAAUUUGACUUCCUCUUUUCCUAAUUGAAUACCCUUUAUUUCCUUCUCCUGC
CUAAUUGCCCUGGCCAGAACUUCCAACACUAUGUUGAAUAGGAGUGGUGAGAGAGGGCAUCCCUGUCUUGUGCCAGUUUU
CAAAGGGAAUGCUUCCAGUUUUUGCCCAUUCACUAUGAUAUUGGCUGUGGCUUUGUCAUAGAUAGCUCUUAUUAUUUUGA
AAUAUGUUCCAUCAAUACCUAAUUUAUUGAGAGUUUUUAGCAUGAUGUGUUGUUGAAUUUUGUCAAAGGCUUUUUCUGCA
UCUAUUGAGAUAAUCAUGUGGUUUUUGUCUUUGGAUCUGUUUAUAUGCUGGAUUACAUUUAUUGAUUUGCGUAUAUUGAA
CCAGCCUUGCAUCCUAGGGAUGAAGCCCACAUGAUCAUGGUGGAUAAGCUUUUUGAUGUGCUGCUGGAUUCGGUUUGCCA
GUAUUUUAUUGAGGAUUUUUGCAUCAAUGUUCAUCAAGGAUAUUGGUCUAAAAUUCUCUUUUUUGGUGUGUCUCUGCCCA
GCUUUGGUAUCAGGAUGAUGUUGGCUUCAUAAAAUGAGUUAGGGAGGAUUCCCUCUUUUUCUAUUGAUUGGAAUAGUUUC
AGAAGGAAUGGUACCAGUUCCUCUUUGUACCUCUGGAGAAUUCGGCUGUGAAUCCAUCUGGUCCUGGACUCUCUUUGGUU
GGUAAGCUAUUGAUUAUUGCCACAAUUUCAGCUCCUGUUAUUGGUCUAUUCAGAGAUUCAACUUCUUCCUGGUUUAGUCU
UGGGAGAGUGUAUGUGUCAAGGAAUUUAUCCAUUUCUUCUAGAUUUUCUAGUUUAUUUGCGUAGAGGUGUUUGUAGUAAU
CUCUGAUGGUAGUUUGUAUUUCUGUGGGAUCGGUGGUGAUAUCCCCUUUAUCAUUUUUUAUUGCGUCUAUUUGAUUCUUC
UCUUUUUCUUUAUUAGUCUUGCUAGCGGUCUAUAAAUUUUGUUGAUCCUUUCAAAAAACCAGCUCCUGGAUUCAUUAAUU
UUUUGAAGGGUUUUUUGUGUCUCUAUUUCCUUCAGUUCUGCUCUGAUUUUAGUUAUUUCUUGCCUUCUGCUAGCUUUUGA
AUAUGUUUGCUCUUGCUUUUCUAGUUCUUUUAAUUGUGAUGUUAGGGUGUCAAUUUUGGAUCUUUCCUGCUUUCUCUUGU
GGGCAUUUAGUGCUAUAAAUUUCCCUCUACACACUGCUUUGAAUGUGUCCCAGAGGUUCUGGUAUGUUGUGUCUUUGUUC
UUGUUGGUUUCAAAGAACAUCUUUAUUUCUGCCUUCAUUUCGUUAUGUACCCAGUAGUCAUUCAGGAGCAGGUUGUUCAG
UUUCCAUGUAGUUGAGCAGUUUUGAGUGAGAUUCUUAAUCCUGAGUUCUAGUUUGAUUGCACUGUGGUCUGAGAGAUAGU
UUGUUAUAAUUUCUGUUCUUUUACAUUUGCUGAGGAGAGCUUUACUUCCAACUAUGUGGUCGGUUUUGGAAUAGGUGUGG
UGUGGUGCUGAAAAAAAUGUAUAUUCUGUUGAUUUGGGAUGGAGUUCUGUAGAUGUCUAUUAGGUCUGCUUGGUGCAGAG
CUGAGUUCAAUUCCUGGGUAUCCUUGUUGACUUUCUGUCUCGUUGAUCUGUGUACUGUUGACAGUGGGUGUUAAAGUCUC
CCAUUAUUAAUGUGUGGAGUCUAAGUCUCUUUGUAGGUCACUCAGAUGAUUGGCACUUACUGGGCGCUUGGCACUUUCCA
UACUGUGUCAUCGGCAGAUAGCUGCAUGGUUGGUGUUCGUGCUGGGGAAUGGGAAGUUCAUCGGUGGGACAAGGACAAAA
UGCCCCCAUUGCUUUGUUGUGGCUUUAAUCUCCCUUUCGAGGCUGAGCCACAGCGUGCUGUAGGUGGCGCUGCUGUGAAG
CGCAGUACCAGGGUCACACUCCACUCCCAGCUCUGCAGAGGUGGAGAAAGAAUGAAACAUCUCACUCCUGGACUUCCACU
UUCCUGUCACUGUUGGUGUCACCUCUUACUGGAUGUCACAGAGCCCAGCCCCUCCCACCUGUGCCUAGGAAAAGCAGAUG
CCACCUUGGAAUGUGGGGUUUGUGUGUGCAAUUUACUAGCUGGGCAGAGACCAGCAACCUGGAGAGCAGGUGUCUCGUCU
AAGGGGACAGUCACAUUUCACCUCCAGCCACCUGGAGGAAUUUGGGCCUGGUGAUGUCAGAAUUCUUCAAUAAAAGCCUA
AAAUCUAUAUUUUAUGUGCGGUCAUGAGAUCUGUUAAAUGUUAGCAACUUCAGGAAGUUUAAAAAUGCUGUGUGGACCUA
GAAUAGGCAAGUUCUUAAAGGCAGAAAGUGGAAUGCUAGUUUCCAGGGACUGGGGAACAGGGAGGAAUGGGGAGUUCAUG
UUUAAUGGGCACAGAGGUUUUGUUAGGGAUGACGAAAAAGUUCGGGAGAUGGUGAUGGUGAUGGAGAUGGUGAUGGUGAU
GGAGAUGGUGAUGGUGAUGGUGAUGGUGAUGGGUGAUGGUGAUGGUGAUGGUGAUGGUGAUGGAGAUGGUGAUGGUGAUG
GUGAUGGAGAUGGUGAUGGUGAUGGUGAUGGUGAUGGAGAUGGUGAUGGUGAUGGAGAUGGUGAUGGUGAUGGUGAUGGA
GAUGGUGAUGGUGAUGGUGAUGGUGAUGGUGAUGGUGAUGGUGAUGGAGAUGGAGAUGGUGAUGGUGAUGGUUGCCUAAC
AUCAGGAACGUGCUUAAUGCUUCUGAAUUGCACACAAAAAUGGCAAGUUUAAUAUUAUGUGUACUUUAUCACAAUGAAAA
AAGCUGCUGCGUGGGCCAAGUUACUUGUGCAGGUAAUGUUCUGCAGGUGGUUGCCUGCACCUCAGUUGUAGGGUGUCCGU
AGGAUGUGAGGCCAGUCCCCGGGCUUAAUGAUGCUUUAAAUCCUGCCUAGUAUUCAAUUAUUUCUUGUCGCUUAAAAGGC
CUAAUAAAAUUAUGGUCUUAGUUUACAGUGGUAUGAAUGCUUAGCUGUUGGAUUUUAGUAGGAAAGUUCGUCCCUUUUUG
UUUUUAAUUUUGUUUUACAGAUUCACAGGAAUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUAAUGCACAGAAAGUUUCCC
UGGACUCUCUACCCAGUUUCCCCAGUGAUAAUAUCUUGGGUAACAUCCUGUAUACAUUCACAUUGGUGCAUUCCUCAGAG
UUGUCAGAUUUUGCUAGUUUUACGUGCACUUGUGUAUGUGUGUAUUUGCAAUUUUAGCACGUGUAGACUCUUGUAACCAC
UACAAUCAAGUUACAGAACUACACUACCAAGGUUCAUCUUUUUAAAAUCUUUGAUGUUACCUUUUUUGGAACAGUGACCA
UGAGAGGACUUUCCUCCCAAAAUUUUGAAAACUACUGAACCAGAAUAUAGUCUGACACUAAUAGGUAGAAAUUUAACCAA
AGGAGAUUAUGAAGCUCUGCACUUGAGUUAACAAAAUCACUUCUCAGCUUCCAGUUCCAUCUCAGAAGGAAGGAAAAGGG
AUUAAAAAUCCAGAGACCAGAAAAUGGGAGCAAAGUACAAGGUGGUGUAAUCAUUACAGAGGUUUCCUGAUGUUUCCAAG
UCAGUCGUGUGUUGAGCUGCUAAACUCUAAAGUAAUUUUAGGUGGAAUGUUGGAAACAUGCUGCUGAGGUGAUAGAAAGG
AAUCCAUGGUCCUCUGUUAGUUGGAAAGUAUAUGGAAUACUAUAUUCUACAUAAGAUACAAUACUCUCUGUGAGACAAGG
AUAAAGUAGAUUUUGUCAGUGAAAUUGUGACAAGAAUCGCUGAUGGGUUUAGAGCCUAAGUUUGCGAGGAGCACUGGAAG
AAAUUAAGAUUGUUGAGAUUGGAAAGGGUUAGCUAUGGGGGAACAGGAGGAGGUGACUCCAUGACAGACCAAAUAUUCAA
AGGACUGUGUAGAAGAGGAAAAAGACUUUGUUAGGGCUCCAGAGGACAGAGCCAGGAGUCAGACAGGGCCUUGAACUCAA
CCCACCGAGAUCUGCAAACUUUGCAGGAUGCACCAGAUGUCUUGUAGCCAUGGGUCAAGGGGGGACCCUGGGUAAGAGAC
UGUAAUAGAUGACCUCUAAGGCCAUCUCAUGACAUGUGUGAUUAAUGUAUGUACCUGUCCUCUCUUUUUGACAAUUCUAC
AGAUUAUUCAGGACAGGGAGUUGACCAACUGCAAAGAGUGAUUGACACCAUCAAAACCAACCCUGACGACAGAAGAAUCA
UCAUGUGCGCUUGGAAUCCAAGAGGUUGAAAGAACCCCGUCGUCUUCAUUUAUACUAACCAUACUCUUAGAGGGAAGCAA
UCUGGUUUUGUGCAGAGGCACUGAGGGAGGCAGGACCCUGGGCAACUUCCCCCAGCCACAUGGUUGUGUGACGUUGGGCA
AGUCACAUUUUGCUGCACUUUCACCUUCAGAUCAUGAGGUUGGGCCCAGAGGAUUUUUUUUUUUUUUUUUUUUUUUGAGA
CAGAGUUUUGCUCUGUUGCCCAGGCUGGAAUGCAACGGCGUGAUCUUGGCUCACUGUAACCUCUGCCUCCUGGGUUCGAG
UGAUUCUCCUGCCUCAGCCUCCAAGUAGCUGGGAUUACAGCAUGUGCCACCAUGCCUGGCUAAUUUUGUAUUUUUAGUAG
AGACGGGUUCACAUGUUGGUCAGGCUGGUCUUGACUCCUGACCCUCAGAUGAUCUGCCUUGCCUCAGCCUCCCAACCGAG
UGAUCUUAAGUUGUGUAUUAUACUCAUUCUUACACAAAAAGGGCUUUAAAUGCCUAGAAACUACAUGAAGAUGUUAACAU
UUUAAAUGGAAGCAGAUGAAGUUCCAGCUCGCUGCCACCUCACUAACAUUUUUAACAAUUAUAUUGUAAAAUUCAACUCU
ACCAGGGUGUAGAGCCAGGUGUGGUGGCUCACACCUGUAAUUCCAACAACUCCAGAGGCCAAGGCGAGAGGAUCAUUUGA
ACCCACGGAAUUUGAGGCUGUAGUGAGUCAUGAUCACGCCAUUGCACUCCAUCCUGGGCAACAGAGUGAGACCCUGAAUA
UUUAAAAACAACAACAACAACAAAACUCUAUCAGGAUAUCAUAAGUACUUAGAGUGAAAUACUUGCAUCUGUAAUAGAGA
CUUAUUUUUUUUUUUUUUGAGACACAGUCUCACCCUGUUGCCCAGGCUGGAGUGCAGUGGUUUGAUCUCCGCUCACGGCA
ACCUCCAUCUCCCAGGUUCAAGUGAGUUCCCAUUCCUCAGCCCCAGAGCUGGGACCACAGGCGCGCGAAUUUUUGUAUUU
UUAGCAGAGACGGGGUUUCACUAUGUUGGCCAGGCUAGUCUCAAACUCAAGUUGGCCUCAAGUGAUCUGCCCACCCUGGC
GUCCCAGUGUUGGGAUUUCAGGCAUGAGCCACUGUGCCUGGCCAUGUAAUAGAGACUUUUAAUAUAGGAGGGUGUACCAG
AAGCACCAGUUUCCUGUGGCAAACAGAAUUAUUCCUGCUGUAUUUGUAAUUUGGUGCCACGAGGUAGCCCAGAUCCCUUC
AGCUCUGAUGGAAGAGCAUUGCUUCAGCCGUAAAUGGACACCUGCAGAAACCUUGCACCGAUGGAUAGUCUCCCUCAGCU
CCGUGCCAUCGCUGCAGGGGCUGUUAUGGACAUCACUGCAGCCCAGUGGCUCUCUCUCCUGGUCUCCACCAUAUGAGUUG
GCUUCUGUUUCUCUCCUGUUUUACUUUGCCUUUAGCUGUGGUCUUUCAAACCACCAUCCCUCCUUAUCUUCCUCUGCUGG
UUCCUCAGAUCUUCCUCUGAUGGCGCUGCCUCCAUGCCAUGCCCUCUGCCAGUUCUAUGUGGUGAACAGUGAGCUGUCCU
GCCAGCUGUACCAGAGAUCGGGAGACAUGGGCCUCGGUGUGCCUUUCAACAUCGCCAGCUACGCCCUGCUCACGUACAUG
AUUGCGCACAUCACGGGCCUGAAGGUGGGCUGUCUCGGGAAGGGUGACUUGCCAGCCUACCACAUGAGCUCUUCAGUUCU
UUAAUAUGGGAAAACAAAUUGCAGAGUUUAGUCUCUGAUUAGCUUUUAAAUUUGAUAUGUGUAAGUAAGACAUGAACCAG
CUUUUACUUUGAAACCUUCCUUUUCUGGAAGGUUUUCUGGCCCUGUGGUAUAUGCACUAACAGAUCUAUACAGGUUGUUU
GUGAUACAGCUUCUAUGGAUCUUCUCAAAAGCUAUGCUGAGGUUGGGUAUGGUGGCUCAUGCCUGUAAUCCCAGCACUUU
GGAAGACUGAGACAGGAGCAAUUGCUUGAGGUCUGGAGUUCAAUACCAGCCUGGGCAACAUAACAAGAUGCUGUUGCUAC
AAAAAAAUGGAAAAGCUACACUAAAUUAUUUUUUUAAAAAAAGCCUUGCGGUGUCUGCAUAUUCUAAUGUUUUUAAAUGA
UGUUUUAAAGAAUUGAAACUAACAUACUGUUCUGCUUUCUCCCGGUUUAUAGCCAGGUGACUUUAUACACACUUUGGGAG
AUGCACAUAUUUACCUGAAUCACAUCGAGCCACUGAAAAUUCAGGUAAGAAUUAGAUGUUAUACUUUUGGGUUUGGUACC
UUCUCUUGAUAAAAGGUUGACUGUGGAACAGGUAUCUGCUCAAUGCUGUGUCCAAGAUAAAGAUGACUGCUCCAAAUGUG
GGGCUUCAGUUUAGGGAGAAGUGGUGGGCAGGUGGGCAGGACAAGGCAGGCAUCUGCCUCAGCAACCAUGGCACUUAACU
UGUCAGGUGCUGUGAGGUACUAAGCACCAGUACCAGAGAGGGAAGAGCCACAUUCAAGCCAGGGGAUUGUCCAAAAGGAG
GCAUUUUAACUCAUUUUAACUUGAAGGAGAAUUGAAGUGCAAAUGUUUUUCCUUUUCUUUUUUUUUGAGAUGGAGUCUUU
CUCUGUCGGCCAGGCUGGAGUGUGCCGUGGUGCGAUCUCAGCUCACUGCAACCUCCACCUCCCGGGUUCAAGCAAUUCUU
CUGCCUCAGCCUCCCAGGUAGCUGGGAUUACAGGCACAUGCCACCACACCCAGCUAAUUUUUUGUAUUAUUAGUAGAGAU
GGGGUUUCGUCAUGUUGGCCAGGCUGAUCUCAAACUCCUGACUUCAAGUGUACCACCUGCCUCAGCCUCCGAAAGUUCUG
GAAUUACAGGCAUAAGCCACCACCCUGGCCAUAAAUAUUUUUUGUUAAUUUUACAUUAAGUACAAUAUUUAGGUCCAAAC
UUCAAAAGUCUGUUGAAAUCCCUGAAGUUAUAGCAGCCAACAAUUGAUAUGAAAUGGCAAUAAAAAUGUAAGUUCAUCUG
CUUCAUGAGCCUUAAGGAAAAAAACUCAGAACCAGACACUUUUUAGCCCCUUCCAGGUUAGAUCCAGGUUUUAAAAGUUA
UUCCUUUGAGGGAGUUUGGCUGCUUUUGAGUGGAGGUGACUUCAGGCUUAUUCUCUCUGGCUCUCUGCUCUGGUCAUUUU
UAGACAUAGUAAUAGGUUGUGACCUGUCUUCACAUCCUAAUUGCCACUGUCUGUUCAUCCCAGGAAUCCUGGCUUUCAUC
CCUUUCUGUUCACUGUCCAUGCAUGUCAUCUUUCCUUCUUUCUGCCAGGGACCAGAUGGGUUAGGGAUUGUGAAUUCAAG
UAAACGUAGAGCUACUAUGAGUUACAGAUUGACUGUGUUCCUGUCUUUAAUAAAUUUGCCAAGAGUGGUUAUAAGAACUU
ACACCUGAUGAGGCACCAGGCUCCUGAUGCUGUGUAAUGUCACAAAAUACCCCUCACUCUCGAUCUGUGCAAGAGAACAG
CUGGUUGCGCUCCAAUCAUGUUACAUAACCUACGCGAAGGUAUCGACAGGAUCAUACUCCUGUAAAAUAGAACUUUGUUG
AUCACAUCCUGUGUACUUGUUUCACGGACAUGAGGAGCAAUUACAACAGGUCGUACAAUUAUGGCAAAAUAAUGGCCUUA
UUUUGUUUUUAGCUUCAGCGAGAACCCAGACCUUUCCCAAAGCUCAGGAUUCUUCGAAAAGUUGAGAAAAUUGAUGACUU
CAAAGCUGAAGACUUUCAGAUUGAAGGGUACAAUCCGCAUCCAACUAUUAAAAUGGAAAUGGCUGUUUAGGGUGCUUUCA
AAGGAGCUCGAAGGAUAUUGUCAGUCUUUAGGGGUUGGGCUGGAUGCCGAGGUAAAAGUUCUUUUUGCUCUAAAAGAAAA
AGGAACUAGGUCAAAAAUCUGUCCGUGACCUAUCAGUUAUUAAUUUUUAAGGAUGUUGCCACUGGCAAAUGUAACUGUGC
CAGUUCUUUCCAUAAUAAAAGGCUUUGAGUUAACUCACUGAGGGUAUCUGACAAUGCUGAGGUUAUGAACAAAGUGAGGA
GAAUGAAAUGUAUGUGCUCUUAGCAAAAACAUGUAUGUGCAUUUCAAUCCCACGUACUUAUAAAGAAGGUUGGUGAAUUU
CACAAGCUAUUUUUGGAAUAUUUUUAGAAUAUUUUAAGAAUUUCACAAGCUAUUCCCUCAAAUCUGAGGGAGCUGAGUAA
CACCAUCGAUCAUGAUGUAGAGUGUGGUUAUGAACUUUAAAGUUAUAGUUGUUUUAUAUGUUGCUAUAAUAAAGAAGUGU
UCUGCAUUCGUCCACGCUUUGUUCAUUCUGUACUGCCACUUAUCUGCUCAGUUCCUUCCUAAAAUAGAUUAAAGAACUCU
CCUUAAGUAAACAUGUGCUGUAUUCUGGUUUGGAUGCUACUUAAAAGAGUAUAUUUUAGAAAUAAUAGUGAAUAUAUUUU
GCCCUAUUUUUCUCAUUUUAACUGCAUCUUAUCCUCAAAAUAUAAUGACCAUUUAGGAUAGAGUUUUUUUUUUUUUUUUU
UAAACUUUUAUAACCUUAAAGGGUUAUUUUAAAAUAAUCUAUGGACUACCAUUUUGCCCUCAUUAGCUUCAGCAUGGUGU
GACUUCUCUAAUAAUAUGCUUAGAUUAAGCAAGGAAAAGAUGCAAAACCACUUCGGGGUUAAUCAGUGAAAUAUUUUUCC
CUUCGUUGCAUACCAGAUACCCCCGGUGUUGCACGACUAUUUUUAUUCUGCUAAUUUAUGACAAGUGUUAAACAGAACAA
GGAAUUAUUCCAACAAGUUAUGCAACAUGUUGCUUAUUUUCAAAUUACAGUUUAAUGUCUAGGUGCCAGCCCUUGAUAUA
GCUAUUUUUGUAAGAACAUCCUCCUGGACUUUGGGUUAGUUAAAUCUAAACUUAUUUAAGGAUUAAGUAGGAUAACGUGC
AUUGAUUUGCUAAAAGAAUCAAGUAAUAAUUACUUAGCUGAUUCCUGAGGGUGGUAUGACUUCUAGCUGAACUCAUCUUG
AUCGGUAGGAUUUUUUAAAUCCAUUUUUGUAAAACUAUUUCCAAGAAAUUUUAAGCCCUUUCACUUCAGAAAGAAAAAAG
UUGUUGGGGCUGAGCACUUAAUUUUCUUGAGCAGGAAGGAGUUUCUUCCAAACUUCACCAUCUGGAGACUGGUGUUUCUU
UACAGAUUCCUCCUUCAUUUCUGUUGAGUAGCCGGGAUCCUAUCAAAGACCAAAAAAAUGAGUCCUGUUAACAACCACCU
GGAACAAAAACAGAUUUUAUGCAUUUAUGCUGCUCCAAGAAAUGCUUUUACGUCUAAGCCAGAGGCAAUUAAUUAAUUUU
UUUUUUUUUGACAUGGAGUCACUGUCCGUUGCCCAGGCUGCAGUGCAGUGGCGCAAUCUUGGCUCACUGCAACCUCCACC
UCCCAGGUUCAAGUGAUUCUCCUGCCUCAGCCUCCCAUGUAGCUGGGAUCACAGGCACCUGCCACCAUGCCCGGCUAAUU
UUUUGUAUUUUUUGUAGAGACAGGGUUUCACCAUGUUGGCCAGGCUGGUCUCAAACACCUGACCUCAAAUGAUCCACCUG
CCUCAGCCUCCCAAAGUGUUGGGAUUACAGGCGUAAGCCACCAUGCCCAGCCCUGAAUUAAUAUUUUUAAAAUAAGUUUG
GAGACUGUUGGAAAUAAUAGGGCAGAGGAACAUAUUUUACUGGCUACUUGCCAGAGUUAGUUAACUCAUCAAACUCUUUG
AUAAUAGUUUGACCUCUGUUGGUGAAAAUGAGCCAUGAUCUCUUGAACAUGAUCAGAAUAAAUGCCCCAGCCACACAAUU
GUAGUCCAAACUUUUUAGGUCACUAACUUGCUAGAUGGUGCCAGGUUUUUUUGCACAAGGAGUGCAAAUGUUAAGAUCUC
CACUAGUGAGGAAAGGCUAGUAUUACAGAAGCCUUGUCAGAGGCAAUUGAACCUCCAAGCCCUGGCCCUCAGGCCUGAGG
AUUUUGAUACAGACAAACUGAAGAACCGUUUGUUAGUGGAUAUUGCAAACAAACAGGAGUCAAAGCUUGGUGCUCCACAG
UCUAGUUCACGAGACAGGCGUGGCAGUGGCUGGCAGCAUCUCUUCUCACAGGGGCCCUCAGGCACAGCUUACCUUGGGAG
GCAUGUAGGAAGCCCGCUGGAUCAUCACGGGAUACUUGAAAUGCUCAUGCAGGUGGUCAACAUACUCACACACCCUAGGA
GGAGGGAAUCAGAUCGGGGCAAUGAUGCCUGAAGUCAGAUUAUUCACGUGGUGCUAACUUAAAGCAGAAGGAGCGAGUAC
CACUCAAUUGACAGUGUUGGCCAAGGCUUAGCUGUGUUACCAUGCGUUUCUAGGCAAGUCCCUAAACCUCUGUGCCUCAG
GUCCUUUUCUUCUAAAAUAUAGCAAUGUGAGGUGGGGACUUUGAUGACAUGAACACACGAAGUCCCUCUGAGAGGUUUUG
UGGUGCCCUUUAAAAGGGAUCAAUUCAGACUCUGUAAAUAUCCAGAAUUAUUUGGGUUCCUCUGGUCAAAAGUCAGAUGA
AUAGAUUAAAAUCACCACAUUUUGUGAUCUAUUUUUCAAGAAGCGUUUGUAUUUUUUCAUAUGGCUGCAGCAGCUGCCAG
GGGCUUGGGGUUUUUUUGGCAGGUAGGGUUGGGAGG
 (-5380.12)

Data files

   For details of Vienna RNA file formats, see the original documentation
   http://www.tbi.univie.ac.at/~ivo/RNA/

Notes

   None.

References

   None.

Warnings

   None.

Diagnostic Error Messages

   None.

Exit status

   It always exits with status 0.

Known bugs

   None.

See also

                     Program name                   Description
                    einverted      Finds inverted repeats in nucleotide sequences
                    vrnaalifold    RNA alignment folding
                    vrnaalifoldpf  RNA alignment folding with partition
                    vrnacofold     RNA cofolding
   vrnacofoldconc   RNA cofolding with concentrations
                    vrnacofoldpf   RNA cofolding with partitioning
                    vrnadistance   RNA distances
                    vrnaduplex     RNA duplex calculation
                    vrnaeval       RNA eval
                    vrnaevalpair   RNA eval with cofold
                    vrnafold       Calculate secondary structures of RNAs
                    vrnafoldpf     Secondary structures of RNAs with partition
                    vrnaheat       RNA melting
                    vrnainverse    RNA sequences matching a structure
                    vrnaplot       Plot vrnafold output
                    vrnasubopt     Calculate RNA suboptimals

Author(s)

                    This program is an EMBOSS conversion of a program written by Ivo
                    Hofacker as part of his VIENNA package.

                    Although we take every care to ensure that the results of the EMBOSS
                    version are identical to those from the original package, we recommend
                    that you check your inputs give the same results in both versions
                    before publication.

                    Please report all bugs in the EMBOSS version to the EMBOSS bug team,
                    not to the original author.

History

                    Converted (October 2005) by Alan Bleasby

Target users

                    This program is intended to be used by everyone and everything, from
                    naive users to embedded scripts.

Comments
