Bio::Tools::EPCR(3)

NAME
Bio::Tools::EPCR - Parse ePCR output and make features

SYNOPSIS
    # A simple annotation pipeline wrapper for ePCR data
    # assuming ePCR data is already generated in file seq1.epcr
    # and sequence data is in fasta format in file called seq1.fa

    use Bio::Tools::EPCR;
    use Bio::SeqIO;
    my $parser = new Bio::Tools::EPCR(-file => 'seq1.epcr');
    my $seqio = new Bio::SeqIO(-format => 'fasta', -file => 'seq1.fa');
    my $seq = $seqio->next_seq || die("cannot get a seq object from SeqIO");

    while( my $feat = $parser->next_feature ) {
        # add EPCR annotation to a sequence
        $seq->add_SeqFeature($feat);
    }
    my $seqout = new Bio::SeqIO(-format => 'embl');
    $seqout->write_seq($seq);

DESCRIPTION
This object serves as a parser for ePCR data, creating a Bio::SeqFeatureI for each ePCR hit. These can be processed or added as annotation to an existing Bio::SeqI object for the purposes of automated annotation.

FEEDBACK

  Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org            - General discussion
http://bioperl.org/MailList.shtml  - About the mailing lists

  Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web:

  bioperl-bugs@bioperl.org
  http://bugzilla.bioperl.org/

AUTHOR - Jason Stajich
Email jason@bioperl.org

Describe contact details here

APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

  new
 Title   : new
 Usage   : my $epcr = new Bio::Tools::EPCR(-file => $file);
 Function: Initializes a new EPCR parser
 Returns : Bio::Tools::EPCR
 Args    : -fh   => filehandle
           OR
           -file => filename

  next_feature
 Title   : next_feature
 Usage   : $seqfeature = $obj->next_feature();
 Function: Returns the next feature available in the analysis result, or
           undef if there are no more features.
 Example :
 Returns : A Bio::SeqFeatureI implementing object, or undef if there are no
           more features.
 Args    : none