Bio::Tools::Est2Genome(3)

NAME
Bio::Tools::Est2Genome - Parse est2genome output, makes simple Bio::SeqFeature::Generic objects

SYNOPSIS
  use Bio::Tools::Est2Genome;

  my $featureiter = new Bio::Tools::Est2Genome(-file => 'output.est2genome');

  # This is going to be fixed to use the SeqAnalysisI next_feature
  # Method eventually when we have the objects to put the data in
  # properly
  while( my $f = $featureiter->parse_next_gene ) {
   # process Bio::SeqFeature::Generic objects here
  }

DESCRIPTION
This module is a parser for est2genome [EMBOSS] alignments of est/cdna sequence to genomic DNA. This is generally accepted as the best program for predicting splice sites based on est/cdnas*.

This module currently does not try pull out the ungapped alignments (Segment) but may in the future.

* AFAIK

FEEDBACK

  Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org              - General discussion
  http://bioperl.org/MailList.shtml  - About the mailing lists

  Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web:

  bioperl-bugs@bioperl.org
  http://bugzilla.bioperl.org/

AUTHOR - Jason Stajich
Email jason@bioperl.org

Describe contact details here

CONTRIBUTORS
Additional contributors names and emails here

APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

  new
 Title   : new
 Usage   : my $obj = new Bio::Tools::Est2Genome();
 Function: Builds a new Bio::Tools::Est2Genome object
 Returns : an instance of Bio::Tools::Est2Genome
 Args    : -file => 'output.est2genome' or
           -fh   => \*EST2GENOMEOUTPUT
           -genomefirst => 1  # genome was the first input (not standard)

  analysis_method
 Usage     : $sim4->analysis_method();
 Purpose   : Inherited method. Overridden to ensure that the name matches
             /est2genome/i.
 Returns   : String
 Argument  : n/a

  parse_next_gene
 Title   : parse_next_gene
 Usage   : @gene = $est2genome_result->parse_next_gene;
           foreach $exon (@exons) {
               # do something
           }

 Function: Parses the next alignments of the est2genome result file and
           returns the found exons as an array of
           Bio::SeqFeature::SimilarityPair objects. Call
           this method repeatedly until an empty array is returned to get the
           results for all alignments.

           The $exon->seq_id() attribute will be set to the identifier of the
           respective sequence for both sequences.
           The length is accessible via the seqlength()
           attribute of $exon->query() and
           $exon->est_hit().
 Returns : An array (or array reference) of Bio::SeqFeature::SimilarityPair and Bio::SeqFeature::Generic objects
           or Bio::SeqFeature::Gene::GeneStructure
 Args    : flag(1/0) indicating to return Bio::SeqFeature::Gene::GeneStructure or Bio::SeqFeature::SimilarityPair
           defaults to 0

  next_feature
 Title   : next_feature
 Usage   : $seqfeature = $obj->next_feature();
 Function: Returns the next feature available in the analysis result, or
           undef if there are no more features.
 Example :
 Returns : A Bio::SeqFeatureI implementing object, or undef if there are no
           more features.
 Args    : none