Bio::Restriction::Enzyme::MultiSite - A single restriction endonuclease # set up a single restriction enzyme. This contains lots of # information about the enzyme that is generally parsed from a # rebase file and can then be read back use Bio::Restriction::Enzyme; This module is used for restriction enzymes that recogonize more than one site. There are some enzymes that recognize sites that cannot be represented by the ambiguous genetic code. For example, M.PhiBssHII recognizes the sites: ACGCGT,CCGCGG,RGCGCY,RCCGGY, and GCGCGC Each site gets its own object that Bio::Restriction::Enzyme will refer to. Each also correlates with the other sites using the method others which stores references to other objects with alternative sites. In this schema each object within an EnzymeCollection can be checked for matching a sequence.

Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/MailList.shtml - About the mailing lists

Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web: bioperl-bugs@bio.perl.org http://bugzilla.bioperl.org/ Heikki Lehvaslaiho, heikki@ebi.ac.uk Rob Edwards, redwards@utmem.edu Copyright (c) 2003 Rob Edwards. Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All Rights Reserved. This module is free software; you can redistribute it and/or modify it under the same terms as Perl itself. Bio::Restriction::Enzyme, Bio::Restriction::Analysis, Bio::Restriction::EnzymeCollection Methods beginning with a leading underscore are considered private and are intended for internal use by this module. They are not considered part of the public interface and are described here for documentation purposes only.

new
Title : new Function Function : Initializes the enzyme object Returns : The Restriction::Enzyme::MultiSite object Argument :

others
Title : others Usage : $re->others(@others); Function : Gets/Sets the a list of other sites that this enzyme recoginizes Arguments : An array containing the other Bio::Restriction::Enzyme::MultiSite objects. Returns : An array containing the other Bio::Restriction::Enzyme::MultiSite objects.

purge_others
Title : purge_others Usage : $re->purge_references(); Function : Purges the set of references for this enzyme Arguments : Returns :