Bio::CodonUsage::Table - for access to the Codon usage Database at http://www.kazusa.or.jp/codon. use Bio::CodonUsage::Table; use Bio::DB::CUTG; ## get a codon usage table from web database ## my $cdtable = Bio::DB::CUTG->new(-sp => 'Mus musculus' -gc => 1); ## or from local file my $io = Bio::CodonUsage::IO->new(-file=>"file"); my $cdtable= $io->next_data(); ## or create your own from your own sequences ## get a Bio::PrimarySeq compliant object ## # $codonstats is a ref to a hash of codon name /count key-value pairs. my $codonstats = Bio::Tools::SeqUtils->codon_count($my_1ary_Seq_objct); ### the '-data' field must be specified ## ### the '-species' and 'genetic_code' fields are optional my $CUT = Bio::CodonUsage::Table->new(-data =>$codonstats, -species => 'Hsapiens_kinase'); print "leu frequency is ", $cdtable->aa_frequency('LEU'), "\n"; print "freqof ATG is ", $cdtable->codon_rel_frequency('ttc'), "\n"; print "abs freq of ATG is ", $cdtable->codon_abs_frequency('ATG'), "\n"; print "number of ATG codons is ", $cdtable->codon_count('ATG'), "\n"; print "gc content at position 1 is ", $cdtable->get_coding_gc('1'), "\n"; print "total CDSs for Mus musculus is ", $cdtable->cds_count(), "\n"; This class provides methods for accessing codon usage table data. All of the methods at present are simple look-ups of the table or are derived from simple calculations from the table. Future methods could include measuring the codon usage of a sequence , for example, or provide methods for examining codon usage in alignments. Bio::Tools::CodonTable, Bio::WebAgent, Bio::CodonUsage::IO, Bio::DB::CUTG

Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bio.perl.org/MailList.html - About the mailing lists

Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web: bioperl-bugs@bio.perl.org http://bugzilla.bioperl.org/ Richard Adams, Richard.Adams@ed.ac.uk The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new
Title : new Usage : my $cut = Bio::CodonUsage::Table->new(-data => $cut_hash_ref, -species => 'H.sapiens_kinase' -genetic_code =>1); Returns : a reference to a new Bio::CodonUsage::Table object Args : none or a reference to a hash of codon counts. This constructor is designed to be compatible with the output of Bio::Tools::SeqUtils::count_codons() Species and genetic code parameters can be entered here or via the species() and genetic_code() methods separately.

all_aa_frequencies
Title : all_aa_frequencies Usage : my $freq = $cdtable->all_aa_frequencies(); Returns : a reference to a hash where each key is an amino acid and each value is its frequency in all proteins in that species. Args : none

codon_abs_frequency
Title : codon_abs_frequency Usage : my $freq = $cdtable->codon_abs_frequency('CTG'); Purpose : To return the frequency of that codon as a percentage of all codons in the organism. Returns : a percentage frequency Args : a non-ambiguous codon string

codon_rel_frequency
Title : codon_rel_frequency Usage : my $freq = $cdtable->codon_rel_frequency('CTG'); Purpose : To return the frequency of that codon as a percentage of codons coding for the same amino acid. E.g., ATG and TGG would return 100 as those codons are unique. Returns : a percentage frequency Args : a non-ambiguous codon string

codon_count
Title : codon_count Usage : my $count = $cdtable->codon_count('CTG'); Purpose : To obtain the absolute number of the codons in the organism. Returns : an integer Args : a non-ambiguous codon string

get_coding_gc
Title : get_coding_gc Usage : my $count = $cdtable->get_coding_gc(1); Purpose : To return the percentage GC composition for the organism at codon positions 1,2 or 3, or an average for all coding sequence ('all'). Returns : a number (%-age GC content) or 0 if these fields are undefined Args : 1,2,3 or 'all'.

set_coding_gc
Title : set_coding_gc Usage : my $count = $cdtable->set_coding_gc(-1=>55.78); Purpose : To set the percentage GC composition for the organism at codon positions 1,2 or 3, or an average for all coding sequence ('all'). Returns : void Args : a hash where the key must be 1,2,3 or 'all' and the value the %age GC at that codon position..

species
Title : species Usage : my $sp = $cut->species(); Purpose : Get/setter for species name of codon table Returns : Void or species name string Args : None or species name string

genetic_code
Title : genetic_code Usage : my $sp = $cut->genetic_code(); Purpose : Get/setter for genetic_code name of codon table Returns : Void or genetic_code id, 1 by default Args : None or genetic_code id, 1 by default if invalid argument.

cds_count
Title : cds_count Usage : my $count = $cdtable->cds_count(); Purpose : To retrieve the total number of CDSs used to generate the Codon Table for that organism. Returns : an integer Args : none (if retrieving the value) or an integer( if setting ).

aa_frequency
Title : aa_frequency Usage : my $freq = $cdtable->aa_frequency('Leu'); Purpose : To retrieve the frequency of an amino acid in the organism Returns : a percentage Args : a 1 letter or 3 letter string representing the amino acid