Bio::Tools::Phylo::PAML - Parses output from the PAML programs codeml, baseml, basemlg, codemlsites and yn00 #!/usr/bin/perl -Tw use strict; use Bio::Tools::Phylo::PAML; # need to specify the output file name (or a fh) (defaults to # -file => "codeml.mlc"); also, optionally, the directory in which # the other result files (rst, 2ML.dS, etc) may be found (defaults # to "./") my $parser = new Bio::Tools::Phylo::PAML (-file => "./results/mlc", -dir => "./results/"); # get the first/next result; a Bio::Tools::Phylo::PAML::Result object, # which isa Bio::SeqAnalysisResultI object. my $result = $parser->next_result(); # get the sequences used in the analysis; returns Bio::PrimarySeq # objects (OTU = Operational Taxonomic Unit). my @otus = $result->get_seqs(); # codon summary: codon usage of each sequence [ arrayref of { # hashref of counts for each codon } for each sequence and the # overall sum ], and positional nucleotide distribution [ arrayref # of { hashref of frequencies for each nucleotide } for each # sequence and overall frequencies ]: my ($codonusage, $ntdist) = $result->get_codon_summary(); # example manipulations of $codonusage and $ntdist: printf "There were %d %s codons in the first seq (%s)\n", $codonusage->[0]->{AAA}, 'AAA', $otus[0]->id(); printf "There were %d %s codons used in all the sequences\n", $codonusage->[$#{$codonusage}]->{AAA}, 'AAA'; printf "Nucleotide %c was present %g of the time in seq %s\n", 'A', $ntdist->[1]->{A}, $otus[1]->id(); # get Nei & Gojobori dN/dS matrix: my $NGmatrix = $result->get_NGmatrix(); # get ML-estimated dN/dS matrix, if calculated; this corresponds to # the runmode = -2, pairwise comparison usage of codeml my $MLmatrix = $result->get_MLmatrix(); # These matrices are length(@otu) x length(@otu) "strict lower # triangle" 2D-matrices, which means that the diagonal and # everything above it is undefined. Each of the defined cells is a # hashref of estimates for "dN", "dS", "omega" (dN/dS ratio), "t", # "S" and "N". If a ML matrix, "lnL" will also be defined. printf "The omega ratio for sequences %s vs %s was: %g\n", $otus[0]->id, $otus[1]->id, $MLmatrix->[0]->[1]->{omega}; # with a little work, these matrices could also be passed to # Bio::Tools::Run::Phylip::Neighbor, or other similar tree-building # method that accepts a matrix of "distances" (using the LOWTRI # option): my $distmat = [ map { [ map { $$_{omega} } @$_ ] } @$MLmatrix ]; # for runmode's other than -2, get tree topology with estimated # branch lengths; returns a Bio::Tree::TreeI-based tree object with # added PAML parameters at each node my $tree = $result->get_tree(); for my $node ($tree->get_nodes()) { # inspect the tree: the "t" (time) parameter is available via # $node->branch_length(); all other branch-specific parameters # ("omega", "dN", etc.) are available via $node->param('omega'); } # get any general model parameters: kappa (the # transition/transversion ratio), NSsites model parameters ("p0", # "p1", "w0", "w1", etc.), etc. my $params = $result->get_model_params(); printf "M1 params: p0 = %g\tp1 = %g\n", $params->{p0}, $params->{p1}; # for NSsites models, obtain arrayrefs of posterior probabilities # for membership in each class for every position; probabilities # correspond to classes w0, w1, ... etc. my @probs = $result->get_posteriors(); # find, say, positively selected sites! if ($params->{w2} > 1) { for (my $i = 0; $i < @probs ; $i++) { if ($probs[$i]->[2] > 0.5) { # assumes model M1: three w's, w0, w1 and w2 (positive selection) printf "position %d: (%g prob, %g omega, %g mean w)\n", $i, $probs[$i]->[2], $params->{w2}, $probs[$i]->[3]; } } } else { print "No positive selection found!\n"; } This module is used to parse the output from the PAML programs codeml, baseml, basemlg, codemlsites and yn00. You can use the Bio::Tools::Run::Phylo::PAML::* modules to actually run some of the PAML programs, but this module is only useful to parse the output.

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Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web: bioperl-bugs@bioperl.org http://bugzilla.bioperl.org/ Email jason@bioperl.org Email amackey@virginia.edu check output from pre 1.12 The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new
Title : new Usage : my $obj = new Bio::Tools::Phylo::PAML(%args); Function: Builds a new Bio::Tools::Phylo::PAML object Returns : Bio::Tools::Phylo::PAML Args : Hash of options: -file, -fh, -dir -file (or -fh) should contain the contents of the PAML outfile; -dir is the (optional) name of the directory in which the PAML program was run (and includes other PAML-generated files from which we can try to gather data)

Implement Bio::AnalysisParserI interface

next_result
Title : next_result Usage : $result = $obj->next_result(); Function: Returns the next result available from the input, or undef if there are no more results. Example : Returns : a Bio::Tools::Phylo::PAML::Result object Args : none