Bio::Search::Hit::BlastHit - Blast-specific subclass of Bio::Search::Hit::GenericHit use Bio::Search::Hit::BlastHit; my $hit = new Bio::Search::Hit::BlastHit(-algorithm => 'blastp'); # See Bio::Search::Hit::GenericHit for information about working with Hits. # TODO: Describe how to configure a SearchIO stream so that it generates # GenericHit objects. This object is a subclass of Bio::Search::Hit::GenericHit and provides some operations that facilitate working with BLAST and PSI-BLAST Hits. For general information about working with Hits, see Bio::Search::Hit::GenericHit.

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Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via email or the web: bioperl-bugs@bioperl.org http://bugzilla.bioperl.org/ Email jason@bioperl.org Email sac@bioperl.org Additional contributors names and emails here The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new
Title : new Usage : my $obj = new Bio::Search::Hit::GenericHit(); Function: Builds a new Bio::Search::Hit::GenericHit object Returns : Bio::Search::Hit::GenericHit Args : See Bio::Search::Hit::GenericHit() for other args. Here are the BLAST-specific args that can be used when creating BlastHit objects: -iteration => integer for the PSI-Blast iteration number -found_again => boolean, true if hit appears in a "previously found" section of a PSI-Blast report.

iteration
Usage : $hit->iteration( $iteration_num ); Purpose : Gets the iteration number in which the Hit was found. Example : $iteration_num = $sbjct->iteration(); Returns : Integer greater than or equal to 1 Non-PSI-BLAST reports will report iteration as 1, but this number is only meaningful for PSI-BLAST reports. Argument : iteration_num (optional, used when setting only) Throws : none See Also : found_again()

found_again
Title : found_again Usage : $hit->found_again; $hit->found_again(1); Purpose : Gets a boolean indicator whether or not the hit has been found in a previous iteration. This is only applicable to PSI-BLAST reports. This method indicates if the hit was reported in the "Sequences used in model and found again" section of the PSI-BLAST report or if it was reported in the "Sequences not found previously or not previously below threshold" section of the PSI-BLAST report. Only for hits in iteration > 1. Example : if( $hit->found_again()) { ... }; Returns : Boolean, true (1) if the hit has been found in a previous PSI-BLAST iteration. Returns false (0 or undef) for hits that have not occurred in a previous PSI-BLAST iteration. Argument : Boolean (1 or 0). Only used for setting. Throws : none See Also : iteration()