Bio::Restriction::Enzyme::MultiCut - A single restriction endonuclease # set up a single restriction enzyme. This contains lots of # information about the enzyme that is generally parsed from a # rebase file and can then be read back use Bio::Restriction::Enzyme; This module defines a restriction endonuclease class where one object represents one of the distinct recognition sites for that enzyme. The method others stores references to other objects with alternative sites. In this schema each object within an EnzymeCollection can be checked for matching a sequence. REBASE report notation "Bsp24I (8/13)GACNNNNNNTGG(12/7)" means: Bsp24I 5' ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^ 3' 3' ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^ 5'

Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/MailList.shtml - About the mailing lists

Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web: bioperl-bugs@bio.perl.org http://bugzilla.bioperl.org/ Heikki Lehvaslaiho, heikki@ebi.ac.uk Rob Edwards, redwards@utmem.edu Copyright (c) 2003 Rob Edwards. Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All Rights Reserved. This module is free software; you can redistribute it and/or modify it under the same terms as Perl itself. Bio::Restriction::Enzyme, Bio::Restriction::Analysis, Bio::Restriction::EnzymeCollection Methods beginning with a leading underscore are considered private and are intended for internal use by this module. They are not considered part of the public interface and are described here for documentation purposes only.

new
Title : new Function Function : Initializes the enzyme object Returns : The Restriction::Enzyme::MultiCut object Argument :

others
Title : others Usage : $re->vendor(@list_of_companies); Function : Gets/Sets the a list of companies that you can get the enzyme from. Also sets the commercially_available boolean Arguments : A reference to an array containing the names of companies that you can get the enzyme from Returns : A reference to an array containing the names of companies that you can get the enzyme from Added for compatibility to REBASE

purge_others
Title : purge_others Usage : $re->purge_references(); Function : Purges the set of references for this enzyme Arguments : Returns :