Mdust - Perl extension for Mdust nucleotide filtering use Bio::Tools::Run::Mdust; my $mdust = Bio::Tools::Run::Mdust->new(); $mdust->run($bio_seq_object); Perl wrapper for the nucleic acid complexity filtering program mdust as available from TIGR (http://www.tigr.org/tdb/tgi/software/). Takes a bioperl primary seq object of type DNA as input. Returns a Bio::Seq object with the low-complexity regions changed to Ns OR a Bio::Seq::RichSeq object with the low-complexity regions identified as a SeqFeature::Generic with primary tag = 'Excluded'. This module uses the environment variable MDUSTDIR to find the mdust program. Set MDUSTDIR to the directory containing the mdust binary (example: if mdust is installed as /usr/local/bin/mdust, set MDUSTDIR to '/usr/local/bin').

EXPORT
None mdust, perl.

Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/MailList.shtml - About the mailing lists

Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: http://bugzilla.bioperl.org/ Donald Jackson (donald.jackson@bms.com) Additional contributors names and emails here The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

new
Title : new Usage : my $mdust = Bio::Tools::Run::Mdust->new( -target => $target_bioseq) Purpose : Create a new mdust object Returns : A Bio::Seq object Args : target - Bio::Seq object for masking - alphabet MUST be DNA. wsize - word size for masking (default = 3) cutoff - cutoff score for masking (default = 28) maskchar - character for replacing masked regions (default = N) coords - boolean - indicate low-complexity regions as Bio::SeqFeature::Generic objects with primary tag 'Excluded', do not change sequence (default 0) tmpdir - directory for storing temporary files debug - boolean - toggle debugging output, do not remove temporary files Note : All of the arguments can also be get/set with their own accessors, such as: my $wsize = $mdust->wsize();

run
Title : run Usage : $mdust->run(); Purpose : Run mdust on the target sequence Args : target (optional) - Bio::Seq object of alphabet DNA for masking Returns : Bio::Seq object (see 'new' for details)

target
Title : target Usage : $mdust->target($bio_seq) Purpose : Set/get the target (sequence to be filtered). Returns : Target Bio::Seq object Args : Bio::Seq object using the DNA alphabet (optional)